A method for quantifying aggression in male Drosophila melanogaster
A method for quantifying aggression in male Drosophila melanogaster
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DOI：
10.1038/nprot.2007.404
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发表时间：
2007-01-01
期刊：
影响因子：
14.8
通讯作者：
Dierick, Herman A.
中科院分区：
文献类型：
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作者：
Dierick, Herman A.
攻击性行为是一种复杂的社会行为，难以衡量。在这里，我们描述了一个简单的方法，在雄性果蝇的侵略性定量分析。传统的攻击性行为的测量依赖于一个领域的背景下，食物领域和女性的因素，诱导或增强侵略。这里描述的方案没有食物领土或女性，使其比大多数用于测量攻击行为的现有方法更简单。多对雄性同时进行测试，以获得平均战斗分数。基于明确的攻击性打斗行为，使用四个参数来量化行为：打斗频率、打斗指数、打斗潜伏期和打斗强度。该测定需要15分钟，在此期间，同时获得20-35对的频率分数。更深入的分析，包括潜伏期，指数和强度，可以在实验的录像记录上进行。该测定具有高度可重复性，并且在简单的设置中需要有限的资源。
Aggressive behavior is a complex social behavior that is difficult to measure. Here, we describe a simple method for the quantitative analysis of aggression in male Drosophila melanogaster. Traditional measurements of aggressive behavior have relied on a territorial context with a food territory and a female as factors that induce or enhance aggression. The protocol described here is devoid of a food territory or a female, making it simpler than most existing methods used to measure aggressive behavior. Multiple pairs of males are tested simultaneously to obtain an average fighting score. Four parameters are used to quantify the behavior: frequency, index, latency and intensity of fighting based on unambiguous offensive fighting behaviors. The assay takes 15 min, during which time a frequency score is obtained for 20-35 pairs simultaneously. More in-depth analysis, including latency, index and intensity, can be performed on the videotaped record of the experiment. The assay is highly reproducible and requires limited resources in a simple setup.