Classification of small cell lung cancer and pulmonary carcinoid by gene expression profiles.
Classification of small cell lung cancer and pulmonary carcinoid by gene expression profiles.
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发表时间：
1999-10
期刊：
影响因子：
11.2
通讯作者：
R. Anbazhagan;T. Tihan;Daniel M. Bornman;James C. Johnston;J. Saltz;Anthony Weigering;S. Piantadosi;E. Gabrielson
中科院分区：
文献类型：
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作者：
R. Anbazhagan;T. Tihan;Daniel M. Bornman;James C. Johnston;J. Saltz;Anthony Weigering;S. Piantadosi;E. Gabrielson
小细胞肺癌是一种常见的肺癌类型，通常被归类为神经内分泌肺癌。利用高密度基因芯片，我们描述了小细胞肺癌的基因表达谱，并将这些表达谱与正常支气管上皮细胞和被归类为良性神经内分泌肿瘤的肺类癌进行了比较。我们发现两个小细胞肺癌细胞系、两个原发小细胞肺癌显微解剖组织样本和培养的支气管上皮细胞的整体表达谱相对相似，这些比较的平均皮尔逊相关系数为0.63。然而，我们发现小细胞肺癌(和支气管上皮细胞)的表达谱与两个肺类癌样本的表达谱惊人地不同，这些比较的平均相关系数为0.20。然后，我们将肺类癌的表达谱与两个浸润性星形细胞脑癌样本(少突胶质瘤和高级别星形细胞瘤)进行比较，发现这四个样本之间的基因表达相似(平均相关系数为0.57)。这些基因表达谱表明，小细胞肺癌与上皮细胞密切相关(并且可能起源于)，而肺类癌与神经脊源性脑瘤有关。更广泛地说，我们的结果表明，广泛的基因表达谱可能揭示肿瘤之间的相似和不同之处，这在传统的形态标准中并不明显。
Small cell lung cancer is a common type of lung cancer that is generally classified within the spectrum of neuroendocrine lung neoplasms. Using high-density cDNA arrays, we profiled gene expression of small cell lung cancers and compared these expression profiles to those of normal bronchial epithelial cells and pulmonary carcinoids, which are classified as benign neuroendocrine tumors. We found the overall expression profiles of two small cell lung cancer cell lines, two microdissected tissue samples of primary small cell lung cancer, and cultured bronchial epithelial cells to be relatively similar to one another, with an average Pearson correlation coefficient for these comparisons of 0.63. However, we found the expression profiles of small cell lung cancers (and bronchial epithelial cells) to be surprisingly dissimilar to those of two samples of pulmonary carcinoid tumors, with an average correlation coefficient for these comparisons of 0.20. We then compared the pulmonary carcinoid expression profiles to those of two samples of infiltrating astrocytic brain cancers (oligodendroglioma and high-grade astrocytoma) and found similarity of gene expression among these four samples (average correlation coefficient, 0.57). These gene expression profiles suggest that small cell lung cancers are closely related to (and possibly derived from) epithelial cells, and that pulmonary carcinoids are related to neural crest-derived brain tumors. More generally, our results suggest that broad profiles of gene expression may reveal similarities and differences between tumors that are not apparent by traditional morphological criteria.