Occurrence of pancreatic lipase-related protein-2 in various species and its. relationship with herbivore diet
Occurrence of pancreatic lipase-related protein-2 in various species and its. relationship with herbivore diet
复制标题
DOI：
10.1016/j.cbpb.2008.01.007
复制
发表时间：
2008-05-01
影响因子：
2.2
通讯作者：
De Caro, Alain
中科院分区：
文献类型：
--
作者：
de Caro, Josiane;Eydoux, Cecilia;De Caro, Alain
研究了10种哺乳动物（人、猪、大鼠、豚鼠、海狸鼠、兔、马、牛、山羊和绵羊）和2种鸟类（鸵鸟和火鸡）胰腺中的经典胰脂肪酶（PL）和胰脂肪酶相关蛋白I和2（PLRP 1 s和PLRP 2）。从脱脂胰腺中纯化脂肪酶，并根据抗人PLRP 2血清的Western印迹分析结果、催化性质和N-末端微测序数据来鉴定。PLRP 2在单胃草食动物（豚鼠、海狸鼠、兔和马）的胰腺中检测到，但在反刍草食动物（牛、山羊和绵羊）的胰腺中未检测到。食肉动物（狗和猫）的胰腺没有任何可检测到的PLRP 2，但含有高水平的PL和PLRP 1。相比之下，杂食性动物（人和大鼠）的胰腺含有PL、PLRP 1和PLRP 2，但猪胰腺除外，其中未检测到PLRP 2。在鸟类（鸵鸟和火鸡）胰腺的情况下，只检测到经典PL。使用磷脂胶束和合成的单分子半乳糖脂膜的PLRP 2的底物特异性进行了研究。与人PLRP 2一样，兔和马PLRP 2是半乳糖脂酶。然而，在多胃食草动物（反刍动物）中，半乳糖脂通过微生物酶促过程（涉及半乳糖脂酶）水解。食肉动物饮食中不含半乳糖脂可以解释为什么在这些物种的胰腺中没有检测到PLRP 2。(C)2008年爱思唯尔公司All rights reserved.
The occurrence of classical pancreatic lipase (PL) and pancreatic lipase-related proteins I and 2 (PLRP1s and 2) in the pancreas of ten mammalian species (humans, pig, rat, guinea pig, coypu, rabbit, horse, ox, goat and sheep) and two bird species (ostrich and turkey) was investigated. The lipases were purified from delipidated pancreas and identified based on the results of Western blotting analysis with anti-human PLRP2 serum, the catalytic properties and N-terminal microsequencing data. PLRP2s were detected in the pancreas of monogastric herbivorous animals (guinea pig, coypu, rabbit and horse) but not in that of ruminant herbivorous animals (ox, goat and sheep). The pancreas of carnivorous animals (dogs and cats) does not have any detectable PLRP2, but contains high levels of PL and PLRP1. By contrast, the pancreas of omnivorous animals (humans and rats) contains PL, PLRP1 and PLRP2, with the exception of porcine pancreas, where no PLRP2 was detected. In the case of bird (ostrich and turkey) pancreases, only classical PL was detected. The substrate specificity of PLRP2s was investigated using phospholipid micelles and synthetic monomolecular galactolipid films. Like human PLRP2, rabbit and horse PLRP2s are galactolipases. In polygastric herbivorous animals (ruminants), however, galactolipids are hydrolyzed via microbial enzymatic processes (involving galactolipases). The absence of galactolipids in carnivorous animals' diet may explain why no PLRP2s were detected here in the pancreas of these species. (C) 2008 Elsevier Inc. All rights reserved.