Adipose Stem Cell-Derived Exosomes Decrease Fatty Infiltration and Enhance Rotator Cuff Healing in a Rabbit Model of Chronic Tears
Adipose Stem Cell-Derived Exosomes Decrease Fatty Infiltration and Enhance Rotator Cuff Healing in a Rabbit Model of Chronic Tears
复制标题
脂肪干细胞衍生的外泌体可减少慢性眼泪兔模型中的脂肪浸润并增强肩袖愈合
DOI：
10.1177/0363546520908847
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发表时间：
2020-04-09
影响因子：
4.8
通讯作者：
He, Yaohua
中科院分区：
文献类型：
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作者：
Wang, Chongyang;Hu, Qingxiang;He, Yaohua
背景：慢性肩袖撕裂(RCT)患者的脂肪渗透和肌腱-骨愈合不良与预后不良有关。脂肪干细胞来源的外切体(ASC-Exos)具有多种生物学效应，可预防急性RCT的肌肉退变。目的：研究ASC-Exos对兔慢性RCT模型脂肪渗透和肌腱-骨愈合的影响。研究设计：对照实验研究。方法：0周时随机分为假手术组(14只)或双侧RCT模型组(21只，冈上肌腱脱离)。6周时，共7只兔接受假手术，7只RCT兔被处死进行脂肪渗出分析。其余14只兔随机分为生理盐水组(7只)和ASC-Exos组(7只)。结果：18周时ASC-Exos组脂肪浸润率(14.01%+/-2.85%)明显低于生理盐水组(21.79%+/-3.07%)(P<；.001)，与修复时间(10.88%+/-2.64%)比较差异无统计学意义(P=.127)。在肌腱-骨愈合方面，ASC-Exos组显示出比生理盐水组更高的组织学评分和更多的修复部位新生的纤维软骨。在生物力学测试方面，ASC-Exos组表现出明显高于生理盐水组的极限载荷、刚度和应力。结论：慢性RCT修复时局部注射ASC-Exos可以阻止脂肪渗透的进展，促进腱-骨愈合，改善生物力学性能。
Background:Fatty infiltration and poor tendon-bone healing in chronic rotator cuff tears (RCTs) are associated with unsatisfactory prognosis. Adipose stem cell-derived exosomes (ASC-Exos), having multiple biological effects, can prevent muscle degeneration in acute RCTs. However, the effects of ASC-Exos on fatty infiltration and tendon-bone healing in chronic RCTs remain unknown.Purpose:To study the effects of ASC-Exos on fatty infiltration and tendon-bone healing in a chronic RCT rabbit model.Study Design:Controlled laboratory study.Methods:At week 0, we randomly allocated 35 rabbits to receive sham surgery (14 rabbits) or establish a bilateral RCT model (21 rabbits, detachment of the supraspinatus tendon). At week 6, a total of 7 rabbits received sham surgery, and 7 rabbits with RCT were sacrificed for fatty infiltration assay. The remaining 14 rabbits with bilateral RCTs were randomly assigned to a saline group (7 rabbits that received local saline injection and rotator cuff repair) or an ASC-Exos group (7 rabbits that received local ASC-Exos injection and rotator cuff repair). At week 18, all rabbits were sacrificed for histological examination and biomechanical testing.Results:At week 18, the ASC-Exos group showed significantly lower fatty infiltration (14.01% +/- 2.85%) compared with the saline group (21.79% +/- 3.07%) (P < .001), and no statistical difference compared with the time of repair (10.88% +/- 2.64%) (P = .127). For tendon-bone healing, the ASC-Exos group showed a higher histological score and more newly regenerated fibrocartilage at the repair site than did the saline group. Regarding biomechanical testing, the ASC-Exos group showed significantly higher ultimate load to failure, stiffness, and stress than the saline group.Conclusion:Local injection of ASC-Exos in chronic RCTs at the time of repair could prevent the progress of fatty infiltration, promote tendon-bone healing, and improve biomechanical properties.