Metabolomics of Supragingival Plaque and Oral Bacteria
Metabolomics of Supragingival Plaque and Oral Bacteria
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DOI：
10.1177/0022034510377792
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发表时间：
2010-12-01
影响因子：
7.6
通讯作者：
Mayanagi, G.
中科院分区：
文献类型：
--
作者：
Takahashi, N.;Washio, J.;Mayanagi, G.
龋齿是由龈上菌斑菌群的糖代谢产生酸而使牙齿表面脱矿引起的。为了阐明糖代谢系统，我们使用CE-MS对龈上菌斑和代表性口腔细菌链球菌和放线菌中的中心碳代谢、EMP途径、戊糖-磷酸途径和TCA循环进行代谢组学研究。龈上菌斑含有中心碳代谢中的所有靶向代谢物，除了戊糖-磷酸途径中的赤藓糖4-磷酸。葡萄糖冲洗后，EMP途径中的葡萄糖6-磷酸、果糖6-磷酸、果糖1，6-二磷酸、二羟丙酮磷酸和丙酮酸以及戊糖-磷酸途径中的6-磷酸葡萄糖酸、核酮糖5-磷酸和景天庚酮糖7-磷酸以及乙酰辅酶A增加。同时，EMP途径中的3-磷酸甘油酸和磷酸烯醇丙酮酸以及TCA循环中的琥珀酸、富马酸和苹果酸减少。在龈上菌斑中观察到的代谢物的这些途径和变化类似于链球菌和放线菌中代谢物谱的整合。
Dental caries is initiated by demineralization of the tooth surface through acid production by sugar metabolism of supragingival plaque microflora. To elucidate the sugar metabolic system, we used CE-MS to perform metabolomics of the central carbon metabolism, the EMP pathway, the pentose-phosphate pathway, and the TCA cycle in supragingival plaque and representative oral bacteria, Streptococcus and Actinomyces. Supragingival plaque contained all the targeted metabolites in the central carbon metabolism, except erythrose 4-phosphate in the pentose-phosphate pathway. After glucose rinse, glucose 6-phosphate, fructose 6-phosphate, fructose 1,6-bisphosphate, dihydroxyacetone phosphate, and pyruvate in the EMP pathway and 6-phosphogluconate, ribulose 5-phosphate, and sedoheptulose 7-phosphate in the pentose-phosphate pathway, and acetyl CoA were increased. Meanwhile, 3-phosphoglycerate and phosphoenolpyruvate in the EMP pathway and succinate, fumarate, and malate in the TCA cycle were decreased. These pathways and changes in metabolites observed in supragingival plaque were similar to the integration of metabolite profiles in Streptococcus and Actinomyces.