A comparative analysis of localized and propagating surface plasmon resonance sensors: The binding of concanavalin a to a monosaccharide functionalized self-assembled monolayer
A comparative analysis of localized and propagating surface plasmon resonance sensors: The binding of concanavalin a to a monosaccharide functionalized self-assembled monolayer
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DOI：
10.1021/ja047118q
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发表时间：
2004-10-06
影响因子：
15
通讯作者：
Van Duyne, RP
中科院分区：
文献类型：
--
作者：
Yonzon, CR;Jeoungf, E;Van Duyne, RP
比较分析了两种光学生物传感器平台的性能：（1）基于平面薄膜金表面的传播表面等离子体共振（SPR）传感器和（2）基于纳米球光刻（NSL）制备的表面限制Ag纳米颗粒的局域表面等离子体共振（LSPR）传感器。选择伴刀豆球蛋白A（ConA）与甘露糖官能化的自组装单分子膜（SAMS）的结合来突出实时角位移SPR和波长位移LSPR生物传感器的响应之间的相似性和差异。在实时结合研究中的缔合阶段期间，SPR和LSPR传感器都表现出定性相似的信号与时间曲线。然而，在解离阶段，SPR传感器显示出比LSPR传感器大约5倍的信号损失。一组全面的非特异性结合研究表明，这种信号差异不是更大的非特异性结合的LSPR传感器的结果，而是银纳米粒子的纳米级结构的系统功能。具有较大的纵横比的银纳米粒子表现出较大的解离相响应比那些具有较小的纵横比。有限元电动力学的基础上的理论分析表明，这是从周围的Ag纳米粒子的尺寸可比的ConA分子的电磁场的特征衰减长度的结果。最后，一个基本的（2 × 1）多路复用版本的LSPR碳水化合物传感芯片，以探测同时结合刀豆蛋白A甘露糖和半乳糖功能化的SAMS已被证明。
A comparative analysis of the properties of two optical biosensor platforms: (1) the propagating surface plasmon resonance (SPR) sensor based on a planar, thin film gold surface and (2) the localized surface plasmon resonance (LSPR) sensor based on surface confined Ag nanoparticles fabricated by nanosphere lithography (NSL) are presented. The binding of Concanavalin A (ConA) to mannose-functionalized self-assembled monolayers (SAMS) was chosen to highlight the similarities and differences between the responses of the real-time angle shift SPR and wavelength shift LSPR biosensors. During the association phase in the real-time binding studies, both SPR and LSPR sensors exhibited qualitatively similar signal vs time curves. However, in the dissociation phase, the SPR sensor showed an approximately 5 times greater loss of signal than the LSPR sensor. A comprehensive set of nonspecific binding studies demonstrated that this signal difference was not the consequence of greater nonspecific binding to the LSPR sensor but rather a systematic function of the Ag nanoparticle's nanoscale structure. Ag nanoparticles with larger aspect ratios showed larger dissociation phase responses than those with smaller aspect ratios. A theoretical analysis based on finite element electrodynamics demonstrates that this results from the characteristic decay length of the electromagnetic fields surrounding Ag nanoparticles being of comparable dimensions to the ConA molecules. Finally, an elementary (2 x 1) multiplexed version of an LSPR carbohydrate sensing chip to probe the simultaneous binding of ConA to mannose and galactose-functionalized SAMS has been demonstrated.