Transglutaminase forms midkine homodimers in cerebellar neurons and modulates the neurite-outgrowth response.
Transglutaminase forms midkine homodimers in cerebellar neurons and modulates the neurite-outgrowth response.
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转谷氨酰胺酶在小脑神经元中形成中期因子同型二聚体并调节神经突生长反应。
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发表时间：
1996
期刊：
影响因子：
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通讯作者：
L. Haynes
中科院分区：
文献类型：
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作者：
S. Mahoney;M. Perry;A. Seddon;P. Bőhlen;L. Haynes
Midkine是大鼠脑神经细胞中2型转谷氨酰胺酶交联蛋白的一个重要的酰基供体底物。转谷氨酰胺酶2型和midkine免疫反应性在发育中的小脑皮质中是局部共定位的。在发育为小脑皮层的胚胎背菱形唇中存在单体midkine。一个高分子量（29- 30kda）的midkine在出生后小脑发育过程中出现。在培养的小脑皮质中间神经元中存在高分子量midkine取决于培养条件。转谷氨酰胺酶主要催化midkine的钙依赖性交联成29- 30kda二聚体。在转谷氨酰胺酶灭活剂的存在下，midkine的二聚体形成在体外和培养的神经元中减少。与单独镀在单体midkine上的神经元相比，镀在先前交联的midkine上的神经元表现出更大的生长锥和增强的神经突生长。
Midkine is a prominent acyl donor substrate for the protein cross-linking enzyme transglutaminase type 2 in rat brain neurons. Transglutaminase type 2 and midkine immunoreactivity are regionally colocalized in developing cerebellar cortex. Monomeric midkine is present in the embryonic dorsal rhombic lip which gives rise to the cerebellar cortex. A high-molecular weight (29-30 kDa) midkine appears during postnatal cerebellar development. The presence of the high-molecular weight midkine in cultured cerebellar cortical interneurons is dependent upon culture conditions. Transglutaminase catalyzes the calcium-dependent cross-linking of midkine predominantly into 29-30 kDa dimers. Dimer-formation of midkine in vitro and in cultured neurons is reduced in the presence of a transglutaminase inactivator. Neurons plated onto previously cross-linked midkine exhibit larger growth cones and enhanced neurite outgrowth compared to those plated onto monomeric midkine alone.