Experience-dependent changes in intracellular Cl- regulation in developing auditory neurons
Experience-dependent changes in intracellular Cl- regulation in developing auditory neurons
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DOI：
10.1016/j.neures.2003.10.011
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发表时间：
2004-02-01
影响因子：
2.9
通讯作者：
Nabekura, J
中科院分区：
文献类型：
--
作者：
Shibata, S;Kakazu, Y;Nabekura, J
据报道，在一系列CNS神经元中，GABA和甘氨酸反应发生了从去极化到超极化的发育变化，并已证明是由于细胞内Cl-浓度（[Cl-]（i））的发育降低所致。我们研究了[Cl-]（i）在分离的大鼠外侧上级橄榄（LSO）神经元使用膜片钳记录甘氨酸门控Cl-电流和测量细胞内Cl-荧光。在14-16日龄大鼠（P14-P16）的神经元中，在听力开始前接受过单侧或双侧耳蜗消融，[Cl-]（i）没有发育性降低。在同侧和对侧消融的大鼠中未观察到[Cl-]（i）的显著差异。植入士的宁颗粒也防止了[Cl-]i在大多数神经元中的减少。在[Cl-]（i）保持高水平的某些神经元中，缺乏K+-Cl-协同转运体2（KCC 2）mRNA的表达。这些结果表明，LSO神经元中[Cl-]（i）的发育减少依赖于神经元活动，GABA能/甘氨酸能和谷氨酸能传入活动均有助于Cl-调节机制的成熟。(C)2003年爱思唯尔爱尔兰有限公司和日本神经科学学会。All rights reserved.
A developmental change in GABA and glycine responses, from a depolarization to a hyperpolarization, have been reported for a range of CNS neurons, and has been demonstrated to be due to a developmental decrease in the intracellular Cl- concentration ([Cl-](i)). We examined [Cl-](i) in isolated rat lateral superior olive (LSO) neurons using patch-clamp recordings of glycine gated Cl- currents and by measuring intracellular Cl--fluorescence. In neurons from 14-16-day-old rats (P14-P16), which had previously received unilateral or bilateral cochlear ablations before the onset of hearing, there was no developmental decrease in [Cl-](i). No significant differences in [Cl-](i) were observed amongst rats with either ipsi- and contralateral ablations. Implanted strychnine pellets also prevented the decrease in [Cl-]i in most neurons. In some of these neurons in which [Cl-](i) remained high, there was a lack of expression of the K+-Cl- cotransporter 2 (KCC2) mRNA. These results demonstrate that the developmental decrease in [Cl-](i) in LSO neurons is dependent on neuronal activity and that both GABAergic/glycinergic and glutamatergic afferent activity contribute to this maturation of the Cl- regulatory mechanisms. (C) 2003 Elsevier Ireland Ltd and The Japan Neuroscience Society. All rights reserved.