Extracting Cell Stiffness from Real-Time Deformability Cytometry: Theory and Experiment.
Extracting Cell Stiffness from Real-Time Deformability Cytometry: Theory and Experiment.
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DOI：
10.1016/j.bpj.2015.09.006
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发表时间：
2015-11-17
影响因子：
3.4
通讯作者：
Fischer-Friedrich E
中科院分区：
文献类型：
--
作者：
Mietke A;Otto O;Girardo S;Rosendahl P;Taubenberger A;Golfier S;Ulbricht E;Aland S;Guck J;Fischer-Friedrich E
细胞硬度是反映细胞生理和病理变化的敏感指标，在生物学和医学领域有着广泛的应用前景。一种新的方法，实时变形性细胞术，通过将细胞暴露于微流体通道中的剪切流，以高通量探测细胞刚度，从而允许基于单细胞变形性的机械表型分析。然而，观察到的变形的细胞在通道中不仅由细胞刚度，但也取决于细胞大小相对于通道的大小。在这里，我们解开细胞的尺寸和细胞的刚度细胞变形的理论分析方面的流体力学和线弹性理论的相互贡献。在已知弹性的模型球体和生物细胞上进行实时变形性细胞术实验，我们证明了我们的分析模型不仅预测通道内的变形形状，而且还允许量化细胞力学参数。因此，大细胞群体的快速和定量机械取样变得可行。
Cell stiffness is a sensitive indicator of physiological and pathological changes in cells, with many potential applications in biology and medicine. A new method, real-time deformability cytometry, probes cell stiffness at high throughput by exposing cells to a shear flow in a microfluidic channel, allowing for mechanical phenotyping based on single-cell deformability. However, observed deformations of cells in the channel not only are determined by cell stiffness, but also depend on cell size relative to channel size. Here, we disentangle mutual contributions of cell size and cell stiffness to cell deformation by a theoretical analysis in terms of hydrodynamics and linear elasticity theory. Performing real-time deformability cytometry experiments on both model spheres of known elasticity and biological cells, we demonstrate that our analytical model not only predicts deformed shapes inside the channel but also allows for quantification of cell mechanical parameters. Thereby, fast and quantitative mechanical sampling of large cell populations becomes feasible.