A (1→4)-β-mannan-specific monoclonal antibody and its use in the immunocytochemical location of galactomannans
A (1→4)-β-mannan-specific monoclonal antibody and its use in the immunocytochemical location of galactomannans
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DOI：
10.1007/s004250100606
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发表时间：
2001-12-01
期刊：
影响因子：
4.3
通讯作者：
Stone, BA
中科院分区：
文献类型：
--
作者：
Pettolino, FA;Hoogenraad, NJ;Stone, BA
将半乳甘露聚糖与蛋白质载体偶联以制备单克隆抗体。产生的单克隆抗体与不同来源的半乳甘露聚糖以及葡甘露聚糖和半乳葡甘露聚糖结合。对一种单克隆抗体 BGM C6 进行了表征，发现其对 (1→4)-β-连接的吡喃甘露糖基残基具有特异性；它对(1--4)-β-连接的甘露六糖的结合亲和力估计为1x10(-6) M。 BGM C6 用于免疫金标记研究，以定位正常椰子 (Cocos nucifera L.) 和两个不同发育阶段的突变体 makapuno 胚乳壁中的半乳甘露聚糖。每种椰子在成熟和未成熟阶段的抗体标记模式和强度各不相同，表明胚乳壁的半乳甘露聚糖组成存在差异。
Galactomannan was coupled to a protein carrier for the preparation of monoclonal antibodies. The monoclonal antibodies generated bound to galactomannans from different sources as well as to glucomannan and galactoglucomannan. One monoclonal antibody, BGM C6, was characterised and found to be specific for (1-->4)-beta-linked mannopyranosyl residues; it had a bindin g affinity estimated at 1x10(-6) M for the (1--4)-beta-linked mannohexaose. BGM C6 was used in immunogold labelling studies to locate galactomannans in the endosperm walls of normal coconuts (Cocos nucifera L.) and those of the mutant makapuno at two different developmental stages. The pattern and intensity of antibody labelling varied for each type of coconut at the mature and immature stages, indicating differences in the galactomannan composition of the endosperm walls.