A PHYSICAL MAP OF THE GROUP-A STREPTOCOCCAL PYROGENIC EXOTOXIN BACTERIOPHAGE-T12 GENOME
A PHYSICAL MAP OF THE GROUP-A STREPTOCOCCAL PYROGENIC EXOTOXIN BACTERIOPHAGE-T12 GENOME
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DOI：
10.1007/bf00337813
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发表时间：
1983-01-01
期刊：
影响因子：
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通讯作者：
SCHLIEVERT, PM
中科院分区：
文献类型：
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作者：
JOHNSON, LP;SCHLIEVERT, PM
用限制性内切酶SalI、Pst I和EcoRI构建了A群链球菌噬菌体T12的物理图谱。该图谱循环排列，总长度为36.0 kb [酶]。亚摩尔量的某些限制性片段，一些非常精确的分子量和一些异质分子量，观察。这一观察结果，连同映射数据，表明DNA包装开始在一个精确的网站上的多联体前体，并进行了有限数量的轮。
A physical map of group A streptococcal bacteriophage T12 was constructed with restriction endonucleases SalI, PstI and EcoRI. The map is circularly permuted with a total length of 36.0 kb [kilobases]. Sub-molar quantities of certain restriction fragments, some of very precise MW and some of heterogeneous MW, were observed. This observation, together with mapping data, suggests that DNA packaging is initiated at a precise site on a concatemeric precursor and proceeds for a limited number of rounds.