Radiolabeled cholesteryl ethers: A need to analyze for biological stability before use.
Radiolabeled cholesteryl ethers: A need to analyze for biological stability before use.
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DOI：
10.1016/j.bbrep.2017.10.007
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发表时间：
2018-03
影响因子：
2.7
通讯作者：
Deckelbaum RJ
中科院分区：
文献类型：
--
作者：
Manual Kollareth DJ;Chang CL;Hansen IH;Deckelbaum RJ
放射性标记的胆固醇醚在各种体内外实验中被广泛用作脂蛋白和脂肪乳剂的非代谢示踪剂。由于胆固醇醚在摄取后不会离开细胞，也不会被哺乳动物细胞酶水解，这些化合物可以作为细胞累积摄取标记粒子的标志。我们用[~3H]胆固醇油醚在动物模型上研究了富含甘油三酯的乳剂颗粒的摄取和分布。然而，令人怀疑的意想不到的结果迫使我们分析这些醚的稳定性。我们采用体外、体内和化学模型系统，测试了不同供应商的两种商业标记胆固醇醚--[~3H]胆固醇油醚和[~3H]胆固醇十六烷基醚的稳定性。结果表明，在两种胆固醇醚中，有一种在体外、体内和在碱性水解剂的作用下都能被水解成游离胆固醇。与胆固醇醚不同，游离胆固醇可以重新进入血液循环，导致混乱的结果。另一种乙醚没有被水解成游离胆固醇，仍然是一种稳定的乙醚。因此，放射性标记的胆固醇醚在用于体外或体内实验之前，应分析其生物稳定性。测试了两种可用于商业用途的放射性标记胆固醇醚的稳定性。在体外和体内，一个乙醚被水解成游离胆固醇(Fc)。FC，重新进入循环，在实验中给出了令人怀疑的意外结果。另一种乙醚在所有模型体系中均为非水解物。放射性标记的胆固醇醚在使用前应进行稳定性分析。
Radiolabeled cholesteryl ethers are widely used as non-metabolizable tracers for lipoproteins and lipid emulsions in a variety of in vitro and in vivo experiments. Since cholesteryl ethers do not leave cells after uptake and are not hydrolyzed by mammalian cellular enzymes, these compounds can act as markers for cumulative cell uptakes of labeled particles. We have employed [3H]cholesteryl oleoyl ether to study the uptake and distribution of triglyceride-rich emulsion particles on animal models. However, questionable unexpected results compelled us to analyze the stability of these ethers. We tested the stability of two commercially available radiolabeled cholesteryl ethers - [3H]cholesteryl oleoyl ether and [3H]cholesteryl hexadecyl ether from different suppliers, employing in vitro, in vivo and chemical model systems. Our results show that, among the two cholesteryl ethers tested, one ether was hydrolyzed to free cholesterol in vitro, in vivo and chemically under alkaline hydrolyzing agent. Free cholesterol, unlike cholesteryl ether, can then re-enter the circulation leading to confounding results. The other ether was not hydrolyzed to free cholesterol and remained as a stable ether. Hence, radiolabeled cholesteryl ethers should be analyzed for biological stability before utilizing them for in vitro or in vivo experiments. Tested stability of two commercially available radiolabeled cholesteryl ethers. One ether was hydrolyzed to free cholesterol (FC) in vitro and in vivo. FC, re-entered circulation giving questionable unexpected results in experiments. The other ether was unhydrolyzed in all model systems. Radiolabeled cholesteryl ethers should be analyzed for stability before use.
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DOI：
10.1161/atvbaha.108.182287
发表时间：
2009-04
期刊：
Arteriosclerosis, thrombosis, and vascular biology
影响因子：
--
作者：
Chang CL;Seo T;Matsuzaki M;Worgall TS;Deckelbaum RJ
通讯作者：
Deckelbaum RJ
影响因子：
4.6
作者：
Anderson, Josephine L. C.;Gautier, Thomas;Tietge, Uwe J. F.
通讯作者：
Tietge, Uwe J. F.
DOI：
10.1161/01.atv.9.1.67
发表时间：
1989-01-01
期刊：
ARTERIOSCLEROSIS
影响因子：
--
作者：
AVIRAM, M;WILLIAMS, KJ;DECKELBAUM, RJ
通讯作者：
DECKELBAUM, RJ
影响因子：
2.9
作者：
Ho, YY;Al-Haideri, M;Deckelbaum, RJ
通讯作者：
Deckelbaum, RJ
影响因子：
2.9
作者：
Kartz, Gabriella A.;Holme, Rebecca L.;Sahoo, Daisy
通讯作者：
Sahoo, Daisy