LATE RADIATION RESPONSE OF THE CANINE TRACHEA WITH CHANGE IN DOSE PER FRACTION
LATE RADIATION RESPONSE OF THE CANINE TRACHEA WITH CHANGE IN DOSE PER FRACTION
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DOI：
10.1016/0360-3016(87)90164-7
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发表时间：
1987-11-01
影响因子：
7
通讯作者：
GILLETTE, EL
中科院分区：
文献类型：
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作者：
POWERS, BE;MCCHESNEY, SL;GILLETTE, EL
72 只狗的气管接受 36 至 74 Gy 的照射，每次剂量为 2、3 或 4 Gy。照射后6个月对气管切片进行组织学和形态测量评估，以确定杯状细胞、粘膜下腺、结缔组织和血管的相对百分比。将每种组织成分的百分比相对于总剂量、计算的回归线和获得的等有效剂量绘制，以构建等效应曲线。还进行了表面溃疡概率的概率分析。另一组 32 只狗接受 36、44 或 52 Gy 的 4 Gy 剂量，并在照射后 1、3 和 12 个月对气管进行类似分析。杯状细胞和粘膜下腺随着每个分数组的每个剂量的总剂量的增加而减少，而结缔组织则增加。每部分的剂量越低，剂量反应曲线越浅，并且需要更高的总剂量才能产生等效应。照射后 6 个月时组织的 α/β 比率为：杯状细胞减少 3.5 Gy，表面溃疡概率 4.7 Gy，粘膜下腺减少 4.5 Gy，结缔组织增加 1.8 Gy。杯状细胞和粘膜下腺数量在 1 个月内减少，并在 12 个月时在较高剂量下仍显着减少。尽管 6 个月时脉管系统体积没有剂量反应，但观察到显着的血管周围和内膜纤维化。这项研究表明，高总剂量和大剂量每次都会对气管造成严重损害。获得的相对较低的α/β比率表明这些不利影响是后期影响。每次剂量较低时，可显着减少不良后期效应。这些结果表明，将气管纳入治疗体积的较粗分馏方案可能存在潜在危险。
Seventy-two dogs were given 36 to 74 Gy to the trachea in either 2, 3, or 4 Gy per fraction. Tracheal sections were histologically and morphometrically evaluated 6 months after irradiation to determine the relative percentage of goblet cells, submucosal glands, connective tissue and blood vessels. The percent of each tissue component was plotted against total dose, regression lines calculated and isoeffective doses obtained for construction of isoeffect curves. Probit analysis for probability of surface ulceration also was done. Another group of 32 dogs received either 36, 44, or 52 Gy in 4 Gy fractions and tracheas were similarly analyzed at 1, 3, and 12 months after irradiation. Goblet cells and submucosal glands decreased with increasing total dose in each of the dose per fraction groups while connective tissue increased. Lower doses per fraction had more shallow dose response curves and higher total doses were required to produce an isoeffect. The alpha/beta ratios for tissues at 6 months after irradiation were 3.5 Gy for decrease in goblet cells, 4.7 Gy for probability for surface ulceration, 4.5 Gy for decrease in submucosal glands and 1.8 Gy for increase in connective tissue. Goblet cells and submucosal gland numbers decreased within 1 month and remained significantly decreased at higher doses at 12 months. Although there was no dose reponse for vasculature volume at 6 months, significant perivascular and intimal fibrosis was observed. This study revealed significant damage to the trachea at high total doses and large doses per fraction. The relatively low alpha/beta ratios obtained indicates that these adverse effects are late effects. Significant sparing of the adverse late effects was present at lower doses per fraction. These results indicate that coarser fractionation schemes that include the trachea in the treatment volume could be potentially dangerous.