The use of environmental DNA of fishes as an efficient method of determining habitat connectivity
The use of environmental DNA of fishes as an efficient method of determining habitat connectivity
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DOI：
10.1016/j.ecolind.2015.11.022
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发表时间：
2016-03-01
影响因子：
6.9
通讯作者：
Minamoto, Toshifumi
中科院分区：
文献类型：
--
作者：
Yamanaka, Hiroki;Minamoto, Toshifumi
本研究展示了环境DNA（eDNA）的使用，以确定栖息地的连接性之间的海洋和河流的鱼类迁移。环境DNA是鱼类在水中释放的DNA片段，可作为物种特异性标记，用于检测目标物种的存在或不存在。一个全年的水采样制度，在淀川，日本，进行了确定是否有三个主要的人为障碍的河流上抑制鱼类的迁移，使用种特异性检测的DNA片段从三个目标移民物种，温带鲈鱼，鲈鱼，平头乌鱼，鲻鱼，香鱼，Plecoglossus altivelis altivelis。目标物种的eDNA的存在/不存在与已知的物种季节性迁移模式一致。在大坝上游最靠近河口的地点检测到温带鲈鱼和平头乌鱼的DNA，表明这些物种通过绕过大坝的鱼梯成功地向上游迁移。另一方面，这两个物种的DNA没有检测到从上游侧的两个剩余的水坝，这是没有配备鱼梯。Ayu是三个目标物种中唯一一个在位于淀川源头的琵琶湖拥有内陆种群的物种。在温暖的月份，在淡水地区的大多数地点都检测到了Ayu DNA;然而，在最冷的2月，仅在琵琶湖南端的淀川最上游的地点检测到了eDNA。我们在这个地点检测到的eDNA表明，它来自于在湖中度过冬季的幼香鱼。这些结果表明，这里提出的eDNA分析可以准确地跟踪鱼类在河流中的季节性迁移，展示其应用程序作为一个指标的鱼类栖息地的连接与人为障碍在河流中。eDNA的采样只需要舀满一罐水;因此，它是一种简单，快速，具有成本效益的方法，用于长期监测与河流中屏障建设相关的栖息地连接。(C)2015爱思唯尔有限公司版权所有。
This study demonstrated the use of environmental DNA (eDNA) to determine habitat connectivity for migration of fishes between the sea and river. Environmental DNA is DNA fragments released by fishes in water, which can be used as a species-specific marker of the presence/absence of the target species. A year-round water sampling regime at 15 sites on the Yodo River, Japan, was conducted to determine whether three major man-made barriers on the river inhibited the migration of fishes using species-specific detection of DNA fragments from three target migrant species, temperate seabass, Lateolabrax japonicas, flathead grey mullet, Mugil cephalus, and ayu, Plecoglossus altivelis altivelis. The presence/absence of eDNA from target species was consistent with known patterns of species' seasonal migration. The detection of the DNA of temperate seabass and flathead grey mullet at sites upstream of the dam closest to the river mouth indicated successful upstream migration of these species via a fish ladder bypassing the dam. On the other hand, DNA of these two species was not detected from the upstream side of the two remaining dams, which are not equipped with fish ladders. Ayu is the only species among the three target species with a land-locked population in Lake Biwa located at the headwater of Yodo River. Ayu DNA was detected at most of the sites in the freshwater area during the warm months; however, in the coldest month of February, eDNA was only detected in the uppermost site of Yodo River at the southern tip of Lake Biwa. The eDNA we detected at this site suggests that it was derived from juvenile ayu spending their winter months in the lake. These results suggest that the eDNA analysis presented here can accurately track the seasonal migration of fishes in a river, demonstrating its application as an indicator of habitat connectivity for fishes in association with man-made barriers in a river. The sampling of eDNA involves merely scooping a tank full of water; therefore, it is a simple, rapid, and cost-effective method for long-term monitoring of habitat connectivity associated with the construction of barriers in a river. (C) 2015 Elsevier Ltd. All rights reserved.