Identifying Schistosoma japonicum excretory/secretory proteins and their interactions with host immune system.
Identifying Schistosoma japonicum excretory/secretory proteins and their interactions with host immune system.
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日本血吸虫排泄/分泌蛋白的鉴定及其与宿主免疫系统的相互作用
DOI：
10.1371/journal.pone.0023786
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Wu Z
中科院分区：
文献类型：
--
作者：
Liao Q;Yuan X;Xiao H;Liu C;Lv Z;Zhao Y;Wu Z
日本血吸虫是血吸虫病的主要病原体。据报道，日本血吸虫在其生命周期中分泌和排泄的大量蛋白质对其在终宿主体内的感染和生存至关重要。这些蛋白质可作为疫苗或药物靶点的理想候选物。在这项工作中，我们分析了日本血吸虫的蛋白质序列，发现与日本血吸虫中的其他蛋白质相比，分泌/排泄（ES）蛋白质通常更长，更有可能是稳定的且为酶，更有可能包含免疫相关结合肽，并且更有可能参与调节和代谢过程。基于ES蛋白和非ES蛋白之间的序列差异，我们训练了一个支持向量机（SVM），其准确性比现有方法高得多。利用这个支持向量机，我们在日本血吸虫中鉴定出191种新的ES蛋白，并进一步预测了这些ES蛋白与人类免疫蛋白之间的7种潜在相互作用。我们的研究结果有助于理解血吸虫病的发病机制，并可作为发现抗血吸虫疫苗或药物靶点的新资源。
Schistosoma japonicum is a major infectious agent of schistosomiasis. It has been reported that large number of proteins excreted and secreted by S. japonicum during its life cycle are important for its infection and survival in definitive hosts. These proteins can be used as ideal candidates for vaccines or drug targets. In this work, we analyzed the protein sequences of S. japonicum and found that compared with other proteins in S. japonicum, excretory/secretory (ES) proteins are generally longer, more likely to be stable and enzyme, more likely to contain immune-related binding peptides and more likely to be involved in regulation and metabolism processes. Based on the sequence difference between ES and non-ES proteins, we trained a support vector machine (SVM) with much higher accuracy than existing approaches. Using this SVM, we identified 191 new ES proteins in S. japonicum, and further predicted 7 potential interactions between these ES proteins and human immune proteins. Our results are useful to understand the pathogenesis of schistosomiasis and can serve as a new resource for vaccine or drug targets discovery for anti-schistosome.
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影响因子：
2.7
作者：
Engels D;Chitsulo L;Montresor A;Savioli L
通讯作者：
Savioli L
影响因子：
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作者：
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影响因子：
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影响因子：
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作者：
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