In vivo formation of glutamyl-tRNAGln in Escherichia coli by heterologous glutamyl-tRNA synthetases
In vivo formation of glutamyl-tRNAGln in Escherichia coli by heterologous glutamyl-tRNA synthetases
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DOI：
10.1016/s0014-5793(03)01460-1
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发表时间：
2004-01-16
期刊：
影响因子：
3.5
通讯作者：
Orellana, O
中科院分区：
文献类型：
--
作者：
Núñez, HN;Lefimil, C;Orellana, O
存在两种类型的谷氨酰-tRNA合成酶：识别酶（D-GluRS）仅形成Glu-tRNA（Glu），而非识别酶（ND-GluRS）也合成Glu-tRNA（Gln），这是许多生物体（但不在大肠杆菌中）蛋白质合成所需的中间体。测试补充热敏E. coli gltX突变体，并抑制E. colitrpA 49错义突变体中，我们检测了异源gltX基因的性质。氧化亚铁硫杆菌GluRS 1和枯草芽孢杆菌Q373 R GluRS形成Glu-tRNA（Glu），A.氧化亚铁酶和幽门螺杆菌GluRS 2在大肠杆菌中形成Glu-tRNA（Gln）。大肠杆菌中的表达。(C)2003年欧洲生物化学学会联合会。Elsevier B. V.出版，保留所有权利。
Two types of glutamyl-tRNA synthetase exist: the discriminating enzyme (D-GluRS) forms only Glu-tRNA(Glu), while the non-discriminating one (ND-GluRS) also synthesizes Glu-tRNA(Gln), a required intermediate in protein synthesis in many organisms (but not in Escherichia colt). Testing the capacity to complement a thermosensitive E. coli gltX mutant and to suppress an E. coli trpA49 missense mutant we examined the properties of heterologous gltX genes. We demonstrate that while Acidithiobacillus ferrooxidans GluRS1 and Bacillus subtilis Q373R GluRS form Glu-tRNA(Glu), A. ferrooxidans and Helicobacter pylori GluRS2 form Glu-tRNA(Gln) in E. coli in vivo. (C) 2003 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.