Bradyrhizobium elkanii Induces Outer Cortical Root Swelling in Soybean
Bradyrhizobium elkanii Induces Outer Cortical Root Swelling in Soybean
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埃氏慢生根瘤菌 (Bradyrhizobium elkanii) 诱导大豆外皮层根肿胀
DOI：
10.1093/oxfordjournals.pcp.a078923
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发表时间：
1995
期刊：
影响因子：
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通讯作者：
K. Minamisawa
中科院分区：
文献类型：
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作者：
Ken;S. Akao;H. Fukuhara;Eiji Tateno;J. Chun;G. Stacey;H. Hara;M. Kubota;T. Asami;K. Minamisawa
用elkaniiUSDA76、USDA94和USDA31接种大豆根系，接种后10 d内观察到根外皮层细胞局部肿胀。这种表型被称为外皮层肿胀（OCS）。接种b的根未见OCS。usda110或USDA122。因此，OCS反应似乎是针对b的。elkanii。为了确定结瘤基因在OCS形成中的作用，我们构建了阳极突变菌株USDA94δNOD。elkaniiUSDA94，其中odd2d1kabc基因被删除。在接种USDA94δNOD的根上观察到OCS响应，表明由noabc基因产物合成的脂质-几丁质结瘤信号不是OCS形成所必需的。
When soybean roots were inoculated withBradyrhizobium elkaniiUSDA76, USDA94 or USDA31, a localized swelling of the root outer cortical cells was observed within 10 days after inoculation. This phenotype was designated outer cortical swelling (OCS). OCS was not observed in roots inoculated withB.japonicumUSDA110 or USDA122. Therefore, the OCS response appears to be specific forB. elkanii. To determine the involvement of the nodulation genes in OCS formation, we constructed anod-mutant, strain USDA94δNOD, ofB. elkaniiUSDA94 in which thenodD2D1KABC genes were deleted. An OCS response was observed on roots inoculated with USDA94δNOD, indicating that the lipo-chitin nodulation signals synthesized by thenodABC gene products are not required for OCS formation.