A self-enhanced renewable electrochemiluminescence biosensing platform for ultrasensitive detection of sialic acid
A self-enhanced renewable electrochemiluminescence biosensing platform for ultrasensitive detection of sialic acid
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用于超灵敏检测唾液酸的自增强可再生电化学发光生物传感平台
DOI：
10.1016/j.electacta.2019.134956
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发表时间：
2019
影响因子：
6.6
通讯作者：
Lin Yanyu
中科院分区：
文献类型：
--
作者：
Fang D;an;Zhang Shupei;Dai Hong;Hong Zhensheng;Lin Yanyu
本文以高效的Ru （II）基复合材料为基础，构建了硼酸亲和可再生电化学发光（ECL）生物传感器，用于唾液酸（SA）的灵敏检测。具体而言，首先将Bi纳米带（Bi NBs）作为共反应物制备自增强Ru(bpy)32+-Bi NBs复合材料，通过缩短电子传输距离和减少能量损失产生强ECL信号。此外，采用hno3辅助合成的具有纳米多孔性质、单晶结构的金红石型tio2介晶（Nss-TiO2-RM）作为支架，增加Ru(bpy)32+和Bi nb的固定化量，进一步增强ECL信号。4-巯基苯基硼酸（4-MPBA）作为仿生识别单元可以代替抗体捕获SA，并且经0.1 M PBS （pH 6）处理后可以获得自清洁电极。在最佳条件下，构建了三明治状可再生ECL生物传感器，对SA在3.5 × 10−5 ~ 350 ng/mL范围内的敏感检测，低检出限为1.17 × 10−5ng/mL。这项工作为其他生物标志物的测定提供了新的思路。
Herein, a boronate-affinity renewable electrochemiluminescence (ECL) biosensor was constructed based on a highly efficient Ru (II)-based composite for the sensitive detection of sialic acid (SA). Specifically, the Bi nanobelts (Bi NBs) was first used as coreactant to prepare a self-enhanced Ru(bpy)32+-Bi NBs composite, producing a strong ECL signal by shortening electronic transmission distance and reducing the energy loss. Additionally, HNO3-assisted synthesized rutile TiO2mesocrystals (Nss-TiO2-RM) with nanoporous nature and single-crystal like structure was employed as scaffold, increasing the immobilization amount of Ru(bpy)32+and Bi NBs, further enhancing ECL signal. Promisingly, 4-Mercaptophenylboronic acid (4-MPBA) as bionic recognition unit could substitute antibody to capture SA, and moreover a self-cleaning electrode could be obtained after treated by 0.1 M PBS (pH 6). Under optimal conditions, a sandwich-like renewable ECL biosensor was constructed for sensitive detection of SA in the range of 3.5 × 10−5to 350 ng/mL with a low detection limit of 1.17 × 10−5ng/mL. This work provided a new thought for other biomarkers determination.