Medium-chain dehydrogenase/reductase and aldo-keto reductase scavenge reactive carbonyls in Synechocystis sp. PCC 6803
Medium-chain dehydrogenase/reductase and aldo-keto reductase scavenge reactive carbonyls in Synechocystis sp. PCC 6803
复制标题
中链脱氢酶/还原酶和醛酮还原酶清除集胞藻中的反应性羰基。
DOI：
10.1002/1873-3468.13003
复制
发表时间：
2018
期刊：
影响因子：
3.5
通讯作者：
Miyake Chikahiro
中科院分区：
文献类型：
--
作者：
Shimakawa Ginga;Kohara Ayaka;Miyake Chikahiro
反应性羰基化合物（RCs）是呼吸和光合作用代谢过程中不可避免产生的，有可能对光合生物造成氧化损伤。此前，我们提出了蓝藻集胞藻中 RC 的清除模型。 PCC 6803（S.6803）。在当前的研究中，我们构建了中链脱氢酶/还原酶（ΔMDR）和醛酮还原酶（ΔAKR）缺陷的突变体，以研究它们对体内 RC 清除的贡献。我们发现，与野生型和 ΔAKR 相比，用脂质衍生的 RC 丙烯醛处理会导致生长抑制并促进 ΔMDR 中更大的蛋白质羰基化。在 ΔMDR 和 ΔAKR 中，光合作用在丙烯醛存在下均受到严重抑制。这些结果表明，这些酶是 S.6803 体内 RC 清除系统的一部分。
Reactive carbonyls (RCs), which are inevitably produced during respiratory and photosynthetic metabolism, have the potential to cause oxidative damage to photosynthetic organisms. Previously, we proposed a scavenging model for RCs in the cyanobacteriumSynechocystissp. PCC 6803 (S. 6803). In the current study, we constructed mutants deficient in the enzymes medium‐chain dehydrogenase/reductase (ΔMDR) and aldo‐keto reductase (ΔAKR) to investigate their contributions to RC scavengingin vivo. We found that treatment with the lipid‐derived RC acrolein causes growth inhibition and promotes greater protein carbonylation in ΔMDR, compared with the wild‐type and ΔAKR. In both ΔMDR and ΔAKR, photosynthesis is severely inhibited in the presence of acrolein. These results suggest that these enzymes function as part of the scavenging systems for RCs in S. 6803in vivo.