Genome-Wide identification and expression analysis of response regulators family genes in Chinese hickory (Carya cathayensis) suggests their potential roles during grafting
Genome-Wide identification and expression analysis of response regulators family genes in Chinese hickory (Carya cathayensis) suggests their potential roles during grafting
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中国山核桃（Carya cathayensis）反应调节家族基因的全基因组鉴定和表达分析表明它们在嫁接过程中的潜在作用
DOI：
10.1007/s00344-022-10898-6
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发表时间：
2023
影响因子：
4.8
通讯作者：
Xiaofei Wang
中科院分区：
文献类型：
--
作者：
Shenchen Tao;Lu Zhao;Jiaqi Mei;Farhat Abbas;Xiaoting Xie;Ying Yang;Qiaoyu Huang;Jiayan Wang;Huwei Yuan;Anket Sharma;Yi He;Bingsong Zheng;Xiaofei Wang
在细胞分裂素双组分系统中，反应调节因子（RR）基因在细胞分裂素信号通路中起着关键作用，并在激活下游基因对细胞分裂素的反应中起着重要作用。然而，山核桃RR基因的特性和表达模式的研究还很少。本研究从中国山核桃中鉴定出17个CcRR基因，其中ACcRR型8个，BCcRR型8个，CCcRR型1个。所有B型和部分A型CcRR（CcRR 5/9a/9 b）均定位于细胞核，而其他A型CcRR（CcRR 3/4/8/16/17）和C型CcRR（CcRR 22）则定位于细胞核和细胞膜。AllCcRRsen编码具有受体结构域的亲水性蛋白。在CcRR的启动子序列上检测到许多植物激素相关的顺式调控元件，qRT-PCR数据证实大多数CcRR基因对各种激素表现出明显的反应，特别是CcRR 1，CcRR 3和CcRR 8。组织特异性表达分析表明，大部分基因在所有组织中均有表达，但也有少数基因具有组织特异性，如胚中的CcRR 4、根中的CcRR 10、果皮中的CcRR 14和种皮中的CcRR 22。在嫁接成功的关键时期，CcRR的表达谱显示，两个CcRR（CcRR 4和CcRR 22）显着下调至原来的不到10%，表明这些基因可能在嫁接过程中起负调控作用。相反，一些基因的表达水平在嫁接的不同阶段上调了2-10倍，这意味着它们可能在嫁接过程中发挥积极作用。本研究结果为进一步了解细胞分裂素调控山核桃嫁接过程的机制提供了依据。
Theresponse regulator(RR) genes play a crucial function in the cytokinin signaling pathway and in activating the downstream genes to respond to cytokinin in the two-component system. However, the characteristics and expression patterns ofRRgenes in Chinese hickory (Carya cathayensis) are still less studied. In this study, 17CcRRgenes were identified from Chinese hickory, including 8 type-ACcRRs, 8 type-BCcRRs, and 1 type-CCcRR. All type-B and some type-A CcRRs (CcRR5/9a/9b) were nucleus-localized, whereas the other type-A CcRRs (CcRR3/4/8/16/17) and type-C CcRRs (CcRR22) were nucleus- and cytomembrane-localized. AllCcRRsencoded hydrophilic proteins with receptor domain. Numerous phytohormone-relatedcis-regulatory elements were detected on the promoter sequences ofCcRRs, and qRT-PCR data verified that the majority ofCcRRgenes showed a noticeable responsiveness to various hormones, particularlyCcRR1,CcRR3, andCcRR8. The tissue-specific expression analysis revealed that the majority of genes were expressed in all tissues, but a few were tissue-specific, such asCcRR4in the embryo,CcRR10in the roots,CcRR14bin the pericarps, andCcRR22in the testae. Expression profiles ofCcRRsduring the critical periods of grafting success showed that twoCcRRs(CcRR4andCcRR22) were significantly down-regulated to less than 10% of the original, indicating that these genes might function as negative regulators in the grafting process. In contrast, the expression levels of some genes were up-regulated by 2–10 times at various stages of the grafting, implying that they might play a positive role in the grafting process. Results from this study will provide the basis for further understanding the mechanisms of cytokinin in regulating the grafting process of Chinese hickory.