A method for the efficient blotting of strongly basic proteins from sodium dodecyl sulfate-polyacrylamide gels to nitrocellulose.
A method for the efficient blotting of strongly basic proteins from sodium dodecyl sulfate-polyacrylamide gels to nitrocellulose.
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一种将十二烷基硫酸钠-聚丙烯酰胺凝胶中的强碱性蛋白质有效印迹到硝酸纤维素上的方法。
DOI：
10.1016/0003-2697(85)90528-7
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发表时间：
1985
影响因子：
2.9
通讯作者：
Kozloff,LM
中科院分区：
文献类型：
--
作者：
Szewczyk,B;Kozloff,LM
本文介绍了一种改进的强碱性蛋白质从十二烷基硫酸钠-聚丙烯酰胺凝胶电泳转移到硝酸纤维素上的方法。使用更多的碱性转移缓冲液和省略转移前的平衡步骤允许强碱性蛋白质从凝胶几乎完全转移到硝酸纤维素，而不降低其他蛋白质的转移效率。
An improved procedure for the electrophoretic transfer of strongly basic proteins from sodium dodecyl sulfate-polyacrylamide gels to nitrocellulose is described. The use of more alkaline transfer buffers and the omission of an equilibration step before the transfer allow for the almost complete transfer of strongly basic proteins from gels to nitrocellulose without lowering the transfer efficiency for other proteins.