Physicochemical properties, metalloproteinases inhibition, and antibiofilm activity of doxycycline-doped dental adhesive
Physicochemical properties, metalloproteinases inhibition, and antibiofilm activity of doxycycline-doped dental adhesive
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DOI：
10.1016/j.jdent.2020.103550
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发表时间：
2021-01-01
影响因子：
4.4
通讯作者：
Franca, Rodrigo
中科院分区：
文献类型：
--
作者：
Freitas, Pedro Henrique;Andre, Carolina Bosso;Franca, Rodrigo
目的：为了评估将多西环素（DOX）掺入商业牙科粘合剂的理化性质、微拉伸粘结强度（mu TBS）、纳米渗漏（NL）、纳米硬度（NH）和杨氏模量（YM）、金属蛋白酶（MMP）抑制和抗氧化膜活性（activity.Methods）：将DOX以0.05、0.1、0.5和1wt%掺入粘合剂中。修复后的牙齿进行了评价亩TBS，NL，NH，和YM后24小时和1年的水存储。变形链球菌的生物膜生长在这些粘合剂上，并测定细菌活力和生物量。结果：DOX浓度为0.5和1wt%时，基质金属蛋白酶的抑制作用降低，转化率降低。DOX的掺入不影响μ TBS和杂化层YM。对照组（不加DOX）在储存1年后具有减少的mu TBS和致密的硝酸银面积。在24小时时，与对照组和1重量% DOX组相比，0.1重量% DOX的混合层NH值增加。储存1年后，与对照组相比，1重量% DOX粘合剂的NH降低。0.5和1重量%浓度的DOX降低了细菌活力和生物膜生物量。结论：在任何浓度下，DOX均能抑制MMP的活性，减少纳米渗漏，并能维持人工老化1年后的树脂-牙本质粘结强度。临床意义：DOX能抑制金属蛋白酶的活性，保持界面粘结强度。该制剂有可能改善粘合剂的临床寿命。
Objectives: To evaluate the incorporation of doxycycline (DOX) into a commercial dental adhesive regarding physicochemical properties, microtensile bond strength (mu TBS), nanoleakage (NL), nanohardness (NH) and Young's modulus (YM), metalloproteinases (MMP) inhibition, and antibiofilm activity.Methods: DOX was incorporated into the adhesive at 0.05, 0.1, 0.5, and 1 wt%. Restored teeth were evaluated for mu TBS, NL, NH, and YM after 24 -hs and 1-year of water storage. Biofilms of Streptococcus mutans were grown on top of these adhesives and determined for bacterial viability and amount of biomass. The inhibitory effect on MMP was analyzed by in situ zymography under confocal microscopy.Results: Adhesives with 0.5 and 1 wt% of DOX presented reduced pH and degree of conversion. The incorporation of DOX did not affect mu TBS and hybrid layer YM. The control group (no DOX) had a decrease in mu TBS and the densest silver nitrate areas after 1-year storage. Hybrid layer NH values increased with 0.1 wt% DOX compared to control and 1 wt% DOX groups, at 24 -hs. After 1-year storage, NH of 1 wt% DOX adhesive decreased compared to the control group. The 0.5 and 1 wt% concentrations of DOX decreased the bacterial viability and the biofilm biomass. Confocal images suggest an increased MMP inhibition proportional to the percentage of DOX.Conclusion: At any concentration, DOX-doped dental adhesives were able to inhibit MMP activity, diminish nanoleakage, and maintain the resin-dentin bond-strength after 1 year of artificial aging.Clinical significance: Doxycycline-doped dental adhesive inhibited metalloproteinases activity and preserved interface bond strength. This formulation has a potential to improve adhesive restorations clinical longevity.