Import into mitochondria of phospholipid hydroperoxide glutathione peroxidase requires a leader sequence
Import into mitochondria of phospholipid hydroperoxide glutathione peroxidase requires a leader sequence
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DOI：
10.1006/bbrc.1996.1525
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发表时间：
1996-10-14
影响因子：
3.1
通讯作者：
Nakagawa, Y
中科院分区：
文献类型：
--
作者：
Arai, M;Imai, H;Nakagawa, Y
采用体外输入系统研究磷脂氢过氧化物谷胱甘肽过氧化物酶（PHGPx）输入线粒体的机制。从大鼠肝脏中分离线粒体，并在25 ℃下与编码23 kDa和20 kDa PHGPx的mRNA体外翻译的[S-35]蛋氨酸标记产物孵育。23-kDa的PHGPx以时间依赖性的方式输入线粒体，并被加工以产生20 kDa形式的PHGPx。没有前导序列的20 kDa形式的PHGPx，与线粒体弱相关，但不输入。与氧化磷酸化解偶联剂的分析表明，在线粒体中的膜电位也需要进口的PHGPx。因此，PHGPx前体中的前导序列似乎是输入线粒体的信号。这是第一份报告表明，PHGPx的前体是通过前导序列的作用输入到线粒体。(C)出版社：Academic Press，Inc.
An in vitro import system was used to characterize the mechanism of import of phospholipid hydroperoxide glutathione peroxidase (PHGPx) into mitochondria. Mitochondria were isolated from rat liver and incubated at 25 degrees C with [S-35]methionine-labeled products of the in vitro translation of mRNA that encoded 23-kDa and 20-kDa PHGPx. 23-kDa PHGPx was imported into mitochondria in a time-dependent manner and was processed to yield the 20-kDa form of PHGPx. The 20-kDa form of PHGPx without a leader sequence, associated weakly with mitochondria but was not imported. An analysis with an uncoupler of oxidative phosphorylation showed that a membrane potential in the mitochondria was also required for the import of PHGPx. It appears, therefore, that the leader sequence in the precursor to PHGPx is the signal for import into the mitochondria. This is the first report to indicate that the precursor to PHGPx is imported into the mitochondria via the action of a leader sequence. (C) 1996 Academic Press, Inc.