SEQUENCES OF THE S1 GENES OF THE 3 SEROTYPES OF REOVIRUS
SEQUENCES OF THE S1 GENES OF THE 3 SEROTYPES OF REOVIRUS
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DOI：
10.1073/pnas.82.1.24
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发表时间：
1985-01-01
影响因子：
11.1
通讯作者：
JOKLIK, WK
中科院分区：
文献类型：
--
作者：
CASHDOLLAR, LW;CHMELO, RA;JOKLIK, WK
对3种血清型呼肠孤病毒的S1基因进行了克隆和测序。S1基因编码Sigma.1蛋白，这种蛋白是针对血清型特异性中和抗体的；它也是呼肠孤病毒血凝素和细胞附着蛋白，是宿主范围/组织特异性的主要决定因素，也是呼肠孤病毒与免疫系统细胞相互作用的性质的主要决定因素。血清型1、2和3型的S1基因长度分别为1458、1442和1416个核苷酸。它们在5‘端有13、13和12个非翻译区，在3’端有188、229和36个核苷酸。它们拥有2个开放阅读框。第一个以弱起始密码子开始，分别延伸到418、399和455个密码子；这是sigma.1蛋白质的预期大小。另一个阅读框以强起始密码子.apprx开始。70个残基，从5‘’末端下游，但只延伸到.apprx。120个密码子，由3个基因的3个同相终止密码子终止。这些短开放阅读框所编码的蛋白质是碱性的。与血清3型S1基因的同源性(分别为5%和9%)相比，1型和2型S1基因之间的同源性(28%)要密切得多。这些数字是基于直接同源计算，调整了25%的随机重合。血清学证据和疏水性图谱表明，1型和2型的.sigma.1蛋白彼此之间的关系要密切得多(.apprx。40%的同源性)，而不是3型(只有.apprx)。20%的同源性)。值得注意的是，在所有其他9个呼肠孤病毒基因中，1和3型呼肠孤病毒基因彼此之间的关系比与2型呼肠孤病毒基因的关系要密切得多。对可能影响这种显着进化模式的机制进行了讨论。
The S1 genes of the 3 serotypes of reovirus were cloned and sequenced. The S1 genes encode protein .sigma.1, the protein against which serotype-specific neutralizing antibodies are directed; it is also the reovirus hemagglutinin and cell-attachment protein and is a major determinant of host range/tissue specificity and of the nature of the interaction of reovirus with cells of the immune system. The S1 genes of serotypes 1, 2 and 3 are 1458, 1442 and 1416 nucleotides long, respectively. They possess untranslated regions 13, 13 and 12 nucleotides long at their 5'' termini and 188, 229 and 36 nucleotides long at their 3'' termini. They possess 2 open reading frames. The 1st starts with a weak initiation codon and extends for 418, 399 and 455 codons, respectively; this is the size expected for the .sigma.1 proteins. The other reading frame starts at a strong initiation codon .apprx. 70 residues downstream from the 5'' terminus but extends for only .apprx. 120 codons, being terminated by 3 in-phase termination codons in all 3 genes. The proteins encoded by these short open reading frames are basic. The serotype 1 and 2 S1 genes are much more closely related to each other (28% homology) than to the serotype 3 S1 gene (5% and 9% homology, respectively). These figures are based on direct homology calculations, adjusted for 25% random coincidence. Serologic evidence and hydrophobicity profiles agree that the .sigma.1 proteins of serotypes 1 and 2 are much more closely related to each other (.apprx. 40% homology) than to that of serotype 3 (only .apprx. 20% homology). The fact that the serotype 1 and 2 S1 genes are much more closely related to each other than to the serotype 3 S1 gene is remarkable since for all other 9 reovirus genes the serotype 1 and 3 genes are much more closely related to each other than to the serotype 2 gene. Mechanisms that may effect this remarkable evolutionary pattern are discussed.