Taurine attenuates lipopolysaccharide-induced disfunction in mouse mammary epithelial cells
Taurine attenuates lipopolysaccharide-induced disfunction in mouse mammary epithelial cells
复制标题
牛磺酸减轻脂多糖诱导的小鼠乳腺上皮细胞功能障碍
DOI：
10.1016/j.cyto.2012.04.009
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发表时间：
2012-07-01
期刊：
影响因子：
3.8
通讯作者：
Zou, Sixiang
中科院分区：
文献类型：
--
作者：
Miao, Jinfeng;Fa, Yanmei;Zou, Sixiang
本研究旨在评估小鼠乳腺上皮细胞的主动防御反应以及牛磺酸对脂多糖（LPS）诱导的小鼠乳腺上皮细胞功能障碍的细胞保护和抗炎作用。(1)用LPS刺激原代培养的小鼠乳腺上皮细胞24 h（终浓度分别为0、5、10、20 μ g/mL）。Western blot结果显示，20 μ g/mL LPS处理组β -酪蛋白分泌显著降低（P < 0.05），一氧化氮（NO）、肿瘤坏死因子- α (tnf - α)、白细胞介素-1 β (1L-1 β)、白细胞介素-6 （IL-6）、乳铁蛋白（LF）和n-乙酰- β - d -氨基葡萄糖酶（NAGase）均显著升高（P < 0.01）。此外，20 μ g/mL LPS显著抑制细胞存活，而5 μ g/mL和10 μ g/mL LPS对细胞存活均无影响。因此，我们选择10 μ g/mL LPS水平来检测牛磺酸对小鼠乳腺上皮细胞的保护作用。(2)分别用0、5、15、45 mmol/L牛磺酸处理小鼠乳腺上皮细胞3 h，再用10 μ g/mL LPS处理24 h。牛磺酸显著降低了LPS诱导的NAGase活性、NO浓度以及tnf - α、IL-1 β、IL-6和LF水平的升高。45 mmol/L牛磺酸显著增加β -酪蛋白分泌，以响应lps诱导的功能障碍。本研究表明，在培养基中添加牛磺酸可显著抑制lps诱导的炎症因子释放，增加乳腺上皮细胞的β -酪蛋白分泌，从而为牛磺酸在预防lps诱导的乳腺上皮细胞功能障碍中的保护作用提供了可能的解释。(C) 2012 Elsevier Ltd.版权所有。
The intent of this study was to evaluate the active defense reaction of mouse mammary epithelial cells and the cytoprotective and anti-inflammatory properties of taurine to lipopolysaccharide (LPS)-induced disfunction in mouse mammary epithelial cells. (1) Primary cultured mouse mammary epithelial cells were stimulated with LPS for 24 h (final concentration = 0, 5, 10, 20 mu g/mL). Western blotting demonstrated a significant decrease in the secretion of beta-casein in the 20 mu g/mL LPS treatment group (P < 0.05), while nitric oxide (NO), tumor necrosis factor-alpha (TNF-alpha), interleukin-1 beta (1L-1 beta), interleukin-6 (IL-6), lactoferrin (LF) and N-acetyl-beta-D-glucosaminidase (NAGase) were all significantly increased following LPS treatment (P < 0.01). Furthermore, cell survival was significantly inhibited after treatment with 20 mu g/mL LPS: however, neither 5 mu g/mL nor 10 mu g/mL LPS had any effect on cell survival. Therefore, a level of 10 mu g/mL LPS was selected to test the protective effect of taurine on mouse mammary epithelial cells. (2) Primary cultured mouse mammary epithelial cells were treated with 0, 5, 15 or 45 mmol/L taurine for 3 h, followed by 10 mu g/mL LPS for 24 h. Taurine significantly attenuated the LPS-induced increase in NAGase activity, NO concentrations and the level of TNF-alpha, IL-1 beta, IL-6 and LF. Taurine at 45 mmol/L markedly increased beta-casein secretion in response to LPS-induced disfunction. This study demonstrated that the addition of taurine to a culture medium significantly inhibited the LPS-induced release of inflammatory factors and increased beta-casein secretion from mammary epithelial cells, thereby providing a possible explanation for the protective effect proposed for taurine in the prevention of LPS-induced disfunction in mammary epithelial cells. (C) 2012 Elsevier Ltd. All rights reserved.