TPA-resistance in Friend erythroleukemia cells: role of membrane lipid fluidity.
TPA-resistance in Friend erythroleukemia cells: role of membrane lipid fluidity.
复制标题
Friend 红白血病细胞中的 TPA 抗性：膜脂流动性的作用。
DOI：
10.1016/s0006-291x(81)80106-4
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发表时间：
1981
影响因子：
3.1
通讯作者：
Weinstein,IB
中科院分区：
文献类型：
--
作者：
Fisher,PB;Cogan,U;Horowitz,AD;Schachter,D;Weinstein,IB
利用1,6-二苯基-1,3,5-己三烯的荧光偏振检测Friend红白血病细胞对促瘤剂12- o -十四烷醇-13-乙酸酯（TPA）敏感或耐药克隆的膜脂动力学。与两个敏感克隆相比，两个抗性克隆产生了更高的荧光各向异性值，表明脂质流动性降低。这种荧光各向异性的差异被半琥珀酸胆甾醇处理的敏感克隆所消除。处理还消除了敏感和抗性克隆之间的另一个表型差异。在TPA存在的塑料培养皿中，细胞粘附性明显降低，这是敏感而非抗性克隆的特性，当前者富含胆固醇酯时。在敏感细胞和耐药细胞之间，没有观察到磷酯与高亲和力饱和受体的结合有显著差异。这些结果暗示膜脂是佛波酯肿瘤促进剂作用的决定因素。
Fluorescence polarization of 1,6-diphenyl-1,3,5-hexatriene was used to examine membrane lipid dynamics in clones of Friend erythroleukemia cells displaying sensitivity or resistance to the tumor promoting agent 12-O-tetradecanoyl-phorbol-13-acetate (TPA). Two resistant clones yielded higher fluorescence anisotropy values, indicative of decreased lipid fluidity, as compared to two sensitive clones. This difference in fluorescence anisotropy was abolished by treatment of the sensitive clones with cholesteryl hemisuccinate. The treatment also removed another phenotypic difference between the sensitive and resistant clones. Cell adherence to plastic culture dishes in the presence of TPA, a property of sensitive but not of resistant clones, was markedly diminished when the former were enriched with the cholesteryl ester. No significant differences were observed in the binding of a phorbol ester to high affinity saturable receptors between sensitive versus resistant cells. These results implicate membrane lipids as determinants of the actions of phorbol ester tumor promoters.