Minimal region of loss at 13q14 in B-cell chronic lymphocytic leukemia
Minimal region of loss at 13q14 in B-cell chronic lymphocytic leukemia
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DOI：
10.1182/blood.v88.8.3109.bloodjournal8883109
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发表时间：
1996-10-15
期刊：
影响因子：
20.3
通讯作者：
Croce, CM
中科院分区：
文献类型：
--
作者：
Bullrich, F;Veronese, ML;Croce, CM
在非随机染色体位点的等位基因丢失被认为标志着肿瘤抑制基因参与人类恶性肿瘤的发病和进展的位置。特别是实体肿瘤，已经发现有多种基因改变导致功能突变的丧失。肿瘤抑制基因也被发现参与淋巴样肿瘤的进展。先前的报道表明，位于视网膜母细胞瘤（RB）和D13S25位点之间的13号染色体长臂上的一个肿瘤抑制基因参与了超过40%的b细胞慢性淋巴细胞白血病（B-CLL）的发病和进展，B-CLL是一种常见的淋巴恶性肿瘤，其分子病因在很大程度上仍然未知。在本研究中，我们报道了一个横跨RB基因和D13S31位点之间约3 cM区域的YAC序列的构建和表征，我们还筛选了60对正常/肿瘤B-CLL样本，在13号染色体上有9个位于RB和D13S25之间的微卫星标记，该分析使我们能够将最小的损失区域缩小到位于206XF12和D13S25标记之间的550 kb片段。(C) 1996年由美国血液病学会出版。
Allelic loss at nonrandom chromosomal sites is thought to mark the position of tumor suppressor genes involved in the pathogenesis and progression of human malignancies. Solid tumors in particular have been found to harbor multiple genetic changes resulting in loss of function mutations. Tumor suppressor genes have also been found to be involved in the progression of lymphoid tumors. Previous reports have suggested the involvement of a tumor suppressor gene located on the long arm of chromosome 13, between the retinoblastoma (RB) and D13S25 loci, in the pathogenesis and or progression of more than 40% of B-cell chronic lymphocytic leukemia (B-CLL), a common lymphoid malignancy whose molecular etiology remains largely unknown. In the present study, we report the construction and characterization of a YAC contig spanning a region of approximately 3 cM between the RB gene and the D13S31 locus, We also screened 60 paired normal/tumor B-CLL samples for allelic loss on chromosome 13 with nine microsatellite markers located between RB and D13S25, This analysis has allowed us to narrow the smallest region of loss to a segment of 550 kb located between the 206XF12 and D13S25 markers, (C) 1996 by The American Society of Hematology.