Depressed primary in vitro antibody response in untreated systemic lupus erythematosus. T helper cell defect and lack of defective suppressor cell function.
Depressed primary in vitro antibody response in untreated systemic lupus erythematosus. T helper cell defect and lack of defective suppressor cell function.
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未经治疗的系统性红斑狼疮的体外初级抗体反应受到抑制。
DOI：
10.1172/jci109827
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发表时间：
1980
期刊：
影响因子：
--
通讯作者：
J. Dormont
中科院分区：
文献类型：
--
作者：
J. Delfraissy;P. Segond;P. Galanaud;C. Wallon;P. Massias;J. Dormont
本文对19例未经治疗的系统性红斑狼疮（SLE）患者、20例正常人和44例正常人外周血淋巴细胞（PBL）的体外抗体反应进行了比较。三硝基苯基聚丙烯酰胺珠（TNP-PAA）用于诱导IgM抗TNP空斑形成细胞。SLE患者表现出明显的抑郁，在大多数情况下几乎没有反应。这不是由于一个不寻常的反应动力学，也不能诱导SLE患者的PBL的预孵育。在SLE患者和正常PBL的共培养物中，除少数例外，前者没有产生抑制作用。在4例患者中，未分级或T细胞耗竭的SLE PBL的抗TNP应答可以通过正常个体的T细胞恢复。相反，在这些患者中的3例中，SLE T细胞不能支持正常B细胞的反应，表明SLE PBL中的T辅助细胞缺陷。伴刀豆球蛋白A（Con A）诱导的抗体应答抑制细胞可通过两种方法测定：（a）在应答SLE患者中，通过将Con A直接加入到TNP-PAA刺激的培养物中;（B）在7名患者中，通过将Con A激活的细胞转移到正常同种异体供体的应答培养物中。在这两种情况下，SLE PBL能够发挥与正常PBL相同程度的抑制作用。
The in vitro antibody response of peripheral blood lymphocytes (PBL) from 19 patients with untreated systemic lupus erythematosus (SLE) was compared with that of 20 control patients and 44 normal subjects. Trinitrophenyl polyacrylamide beads (TNP-PAA) were used to induce IgM anti-TNP plaque-forming cells. SLE patients displayed a markedly depressed, and in most instances virtually absent, response. This was not due to an unusual kinetics of the response; nor could it be induced by preincubation of SLE patients' PBL. In co-cultures of SLE patients and normal PBL, the former, with few exceptions, did not exert a suppressive effect. In four patients the anti-TNP response of either unfractionated or T-depleted SLE PBL could be restored by T cells from a normal individual. Conversely in three of these patients, SLE T cells could not support the response of normal B cells, suggesting a T helper cell defect in SLE PBL. Concanavalin A (Con A)-induced suppressor cells of the antibody response could be assayed by two approaches: (a) in responder SLE patients, by the direct addition of Con A to TNP-PAA-stimulated cultures; (b) in seven patients by transfer of Con A-activated cells to the responding culture of a normal allogeneic donor. In both cases SLE PBL were able to exert a suppressive effect to the same extent as normal PBL.