Induced sputum and bronchoalveolar lavage from patients with hypersensitivity pneumonitis
Induced sputum and bronchoalveolar lavage from patients with hypersensitivity pneumonitis
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DOI：
10.1016/j.rmed.2004.03.007
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发表时间：
2004-10-01
影响因子：
4.3
通讯作者：
Olivieri, D
中科院分区：
文献类型：
--
作者：
D'Ippolito, R;Chetta, A;Olivieri, D
背景与目的：过敏性肺炎（HP）是一种免疫诱导的肺实质炎症，尽管支气管气道也可能受累。本研究的目的是比较新诊断的HP患者和健康人的诱导痰（IS）的细胞谱，并检查IS和BAL的炎性细胞之间的关系。方法：9例HP患者和9例健康志愿者进行了研究。所有受试者均通过吸入高渗盐溶液获得IS。支气管镜检查进行了不同的场合，在所有患者和五个controls.Results：IS耐受性良好，首选BAL的所有科目。HP患者的IS和BAL与正常人相比，细胞总数（P < 0.02和P < 0.001）和淋巴细胞（P < 0.02和P < 0.001）均显著增加，巨噬细胞（P < 0.05和P < 0.001）显著减少。HP患者IS中细胞总数明显高于BAL（P < 0.02）。IS组淋巴细胞百分率明显低于BAL组（P < 0.001）。没有显着的关系，发现总细胞或炎症细胞从IS和相应的从BAL和广泛的限制之间的协议被发现淋巴细胞从IS和BAL.Conclusions：这项研究表明，无论是BAL和IS从新诊断的HP患者包含显着更多的总细胞和淋巴细胞，相比健康受试者。此外，HP患者中的差异细胞计数显示IS和BAL反映了不同的炎症区室。因此，在研究和HP患者的临床监测中，IS可以代表支气管镜检查的补充，但不是替代。（C）2004 Elsevier Ltd.保留所有权利。
Background and Aim: Hypersensitivity pneumonitis (HP) is an immunologically induced inflammation of the lung parenchyma, though bronchial airways may be also involved. The aim of this study was to compare the cellular profiles of induced sputum (IS) in patients with newly diagnosed HP to that of healthy subjects, and to examine the relationship between inflammatory cells from IS and BAL.Methods: Nine HP patients and 9 healthy volunteers were studied. IS was obtained by inhalation of hypertonic saline solution in all subjects. Bronchoscopy was performed on a different occasion in all patients and in five controls.Results: IS was well tolerated and preferred to BAL by all subjects. Both IS and BAL from HP patients showed a significant increase in total cells (P < 0.02 and P < 0.001) and in lymphocytes (P < 0.02 and P < 0.001) and a significant decrease in macrophages (P < 0.05 and P < 0.001), when compared with normal subjects. In HP patients, total cells number in IS was higher than that in BAL (P < 0.02). Moreover, the percentage of lymphocytes was significantly tower in IS than in BAL (P < 0.001). No significant relationship was found between total cells or inflammatory cells from IS and the corresponding ones from BAL and wide limits of agreement were found between lymphocytes from IS and BAL.Conclusions: This study demonstrated that both BAL and IS from newly diagnosed HP patients contained significantly more total cells and lymphocytes, when compared to healthy subjects. Moreover, differential cell counts in HP patients showed that IS and BAL reflected different compartments of inflammation. Thus, IS could represent a complementary, but not alternative toot to bronchoscopy both in research and in the clinical monitoring of HP patients. (C)2004 Elsevier Ltd. All rights reserved.