Estradiol and incubation temperature modulate regulation of steroidogenic factor 1 in the developing gonad of the red-eared slider turtle
Estradiol and incubation temperature modulate regulation of steroidogenic factor 1 in the developing gonad of the red-eared slider turtle
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DOI：
10.1210/en.142.4.1403
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发表时间：
2001-04-01
期刊：
影响因子：
4.8
通讯作者：
Crews, D
中科院分区：
文献类型：
--
作者：
Fleming, A;Crews, D
红耳滑龟在性别决定方面具有遗传上的双潜能，卵的孵化温度决定性腺的性别。在较高的孵化温度下，可能由于雌激素的生物合成增加而产生雌性。外源性雌激素导致卵巢的形成，阻止雌激素的生物合成导致睾丸的发育。在哺乳动物中，类固醇生成因子1（SF-1）调节从胆固醇生物合成雌激素所需的大多数基因。在哺乳动物以及红耳滑龟中，SF-1在性腺发生期间在雄性和雌性中差异表达。为了检测SF-1在温度依赖性性别决定中的可能作用，我们测定了在野生型有机体的性腺发生期间改变性发育的处理后红耳滑龟中SF-1的表达。我们检测了胚胎中的性腺SF-1表达：1）在三种不同温度下孵育，2）在雄性产生温度下用雌激素处理卵后，和3）在雌性产生温度下抑制雌激素生物合成后。我们的研究结果表明，温度和雌激素都位于SF-1的上游，在红耳滑龟的性别决定的监管层次，雌激素直接或间接地调节SF-1的表达。
Red-eared slider turtles are genetically bipotential for sex determination, with incubation temperature of the egg determining gonadal sex. At higher incubation temperatures, females are produced, possibly due to increased biosynthesis of estrogen. Exogenous estrogen causes the formation of ovaries, and prevention of estrogen biosynthesis results in the development of testes. In mammals, steroidogenic factor 1 (SF-l) regulates most genes required for estrogen biosynthesis from cholesterol. In mammals as well as red-eared slider turtles, SF-l is differentially expressed in males and females during gonadogenesis. To examine a possible role for SF-l in temperature- dependent sex determination, we assayed its expression in red-eared slider turtles after treatments that alter sex development during gonadogenesis of the wild-type organism.We examined gonadal SF-1 expression in embryos 1) incubating at three different temperatures, 2) after treating eggs with estrogen at a male-producing temperature, and 3) after inhibition of estrogen biosynthesis at a female-producing temperature. Our findings suggest that both temperature and estrogen lie upstream of SF-1 in a sex-determining regulatory hierarchy in red-eared slider turtles and that estrogen directly or indirectly modulates the regulation of SF-1 expression.