Age-dependence of urinary normal and modified nucleosides in childhood as determined by reversed-phase high-performance liquid chromatography
Age-dependence of urinary normal and modified nucleosides in childhood as determined by reversed-phase high-performance liquid chromatography
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DOI：
10.1016/j.jchromb.2004.10.051
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发表时间：
2005-01-25
影响因子：
3
通讯作者：
Gérard, H
中科院分区：
文献类型：
--
作者：
Liebich, HM;Müller-Hagedorn, S;Gérard, H
修饰核苷已被定性为许多恶性疾病的肿瘤标志物。为了在儿童中使用这些标记物，必须确定健康儿童核苷水平的年龄依赖性，并在诊断决策中予以考虑。在这项研究中，通过反相高效液相色谱法测定了 166 名年龄在 1 天至 19 岁之间的健康儿童和青少年尿液中 12 种正常核苷和修饰核苷的水平，并定义了与年龄相关的参考范围。尿核苷浓度与肌酐浓度相关，因此可以使用随机收集的尿样。儿童尿液中的所有核苷水平随着年龄的增长而降低，在生命的前 4 年内最为明显，各个核苷参考值的年龄依赖性可以通过数学函数 y = b(0) + b(1) (1/x) 以及回归系数 b(0) 和 b(1)、核苷水平 y 和 1 岁至 19 岁之间的年龄 x 来近似。在年幼的儿童中，核苷浓度的变化更加差异化。从生命第一天的低水平开始，所有研究的核苷浓度上升到 1-2 个月龄，此时达到所有年龄段的绝对最大值，然后下降。 (C) 2004 Elsevier B.V. 保留所有权利。
Modified nucleosides have been characterized as tumor markers for a number of malignant diseases. In order to use these markers in children, the age-dependence of the nucleoside levels in healthy children has to be established and taken into account in diagnostic decisions. In this study, the levels of 12 normal and modified nucleosides in urine of 166 healthy children and adolescents with an age between 1 day and 19 years are determined by reversed-phase HPLC, and age-dependent reference ranges are defined. The urinary nucleoside concentrations are related to the creatinine concentrations, which allows the use of randomly collected urine samples. All nucleoside levels in urine of children decrease with age, most pronounced during the first 4 years of life, and the age-dependence of the reference values of the individual nucleosides can be approximated by a mathematical function y = b(0) + b(1) (1/x) with the regression coefficients b(0) and b(1), the nucleoside levels y and the age x between 1 year and 19 years. In the very young children, the shifts in the nucleoside concentrations are more differentiated. Starting with low levels on the first day of life, the concentrations of all studied nucleosides rise up to an age of 1-2 months, when they reach their absolute maximum for all age periods, and then decrease. (C) 2004 Elsevier B.V. All rights reserved.