Salicylic acid interferes with tobacco mosaic virus replication via a novel salicylhydroxamic acid-sensitive mechanism
Salicylic acid interferes with tobacco mosaic virus replication via a novel salicylhydroxamic acid-sensitive mechanism
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DOI：
10.1105/tpc.9.4.547
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发表时间：
1997-04-01
期刊：
影响因子：
11.6
通讯作者：
Carr, JP
中科院分区：
文献类型：
--
作者：
Chivasa, S;Murphy, AM;Carr, JP
水杨酸(SA)可诱导对包括细菌、真菌和病毒在内的所有植物病原体产生抗性，但SA产生病毒抗性的机制尚不清楚。用SA对烟草花叶病毒(TMV)敏感(nn基因型)烟草组织进行SA预处理，可降低接种TMV后病毒RNA和病毒外壳蛋白的积累水平。病毒RNA受到的影响并不相同，这表明SA处理干扰了TMV的复制。水杨基异羟肟酸(SHAM)是一种线粒体交替氧化酶的抑制剂，它既能拮抗SA诱导的nn型烟草对TMV的抗性，也能拮抗SA诱导的Nn型烟草的获得性抗性。SHAM不抑制SA诱导PR-1致病相关蛋白或诱导其对胡萝卜欧文氏菌或灰霉病菌的抗性。这表明SA通过一种新的假敏感信号转导途径(可能涉及交替氧化酶)诱导对TMV的抗性，这与导致对细菌和真菌产生抗性的信号转导途径不同。
Salicylic acid (SA) induces resistance to all plant pathogens, including bacteria, fungi, and viruses, but the mechanism by which SA engenders resistance to viruses is not known. Pretreatment of tobacco mosaic virus (TMV)-susceptible (nn genotype) tobacco tissue with SA reduced the levels of viral RNAs and viral coat protein accumulating after inoculation with TMV. Viral RNAs were not affected equally, suggesting that SA treatment interferes with TMV replication. Salicylhydroxamic acid (SHAM), an inhibitor of the mitochondrial alternative oxidase, antagonized both SA-induced resistance to TMV in nn genotype plants and SA-induced acquired resistance in resistant (NN genotype) tobacco. SHAM did not inhibit induction of the PR-1 pathogenesis-related protein or induction of resistance to Erwinia carotovora or Botrytis cinerea by SA. This indicates that SA induces resistance to TMV via a novel SHAM-sensitive signal transduction pathway (potentially involving alternative oxidase), which is distinct from that leading to resistance to bacteria and fungi.