Simultaneous Determination of Six Components in the 'Jiang-Zhi' Granule by UPLC-MS Analysis
Simultaneous Determination of Six Components in the 'Jiang-Zhi' Granule by UPLC-MS Analysis
复制标题
UPLC-MS同时测定“降脂”颗粒中的六种成分
DOI：
10.1016/s1875-5364(11)60007-1
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发表时间：
2010-11-01
影响因子：
4.6
通讯作者：
Ji Guang
中科院分区：
文献类型：
--
作者：
Lu Yan-Liu;Wang Miao;Ji Guang
目的：建立超高效液相色谱-质谱法同时测定降脂颗粒中6种成分含量的新方法。方法：采用Acquity UPLC BEH C-18色谱柱(2.1 mm×50 mm，1.7微米)；洗脱梯度为甲醇和0.1%甲酸。采用选择离子监测器(SIM)模式检测目标化合物。结果：6种目标化合物的回收率均在97.58%~103.12%之间，在考察的浓度范围内线性关系良好(R-2和GT；0.999 2)，日内、日间精密度均小于3%。结论：建立的方法可用于降脂颗粒中6种主要成分的含量测定。
AIM: A novel and rapid method using an ultra-performance liquid chromatography coupled with mass spectrometry was developed and validated for the simultaneous determination of six constituents in 'Jiang-Zhi' granule. METHODS: An acquity UPLC BEH C-18 column (2.1 mm x 50 nun, 1.7 mu m) was utilized. Methanol and 0.1% formic acid were adopted in the elution gradient. The selective ion monitor (SIM) mode was used to detect the target compounds. RESULTS: The established method showed a good linearity (R-2 > 0.999 2) over the investigated concentration ranges, good inter-day and intra-day precisions (less than 3%) and good recoveries (from 97.58% to 103.12%) for all six target compounds. CONCLUSION: The contents of six main components in 'Jiang-Zhi' granule could be determined using the established method.