Glycosylation of liver acute-phase proteins in pancreatic cancer and chronic pancreatitis
Glycosylation of liver acute-phase proteins in pancreatic cancer and chronic pancreatitis
复制标题
DOI：
10.1002/prca.200900150
复制
发表时间：
2010-04-01
影响因子：
2
通讯作者：
Peracaula, Rosa
中科院分区：
文献类型：
--
作者：
Sarrats, Ariadna;Saldova, Radka;Peracaula, Rosa
目的：急性期蛋白（APP）的糖基化，这是部分由细胞因子调节，可能是不同的疾病，并提供有用的肿瘤标志物。因此，我们检测了胰腺癌（PaC）、慢性胰腺炎（CP）和对照患者血清APP的糖基化。实验设计：使用特异性抗唾液酸刘易斯X抗体和N-聚糖测序，我们测定了α-1-酸性糖蛋白（AGP）、触珠蛋白（HPT）、胎球蛋白（FET）、α-1-抗胰蛋白酶（AT）和转铁蛋白（TRF）的糖基化变化。晚期PaC和CP患者的AGP和CP患者的HPT、FET、AT和TRF检测到唾液酸刘易斯X（Sle（x））水平升高。在晚期PaC和CP的AGP和HPT上以及CP的FET和TRF上检测到N-聚糖分支增加。核心岩藻糖基化结构增加AGP和HPT只有在先进的PaC patients.Conclusions和临床意义：APP SLe（x）和分支的变化可能与炎症反应，因为他们都检测到先进的PaC和CP患者和这些条件引起炎症。相反，APP核心岩藻糖基化的增加可能与癌症相关，这种糖型的存在可能对肿瘤有利。
Purpose: Glycosylation of acute-phase proteins (APP), which is partially regulated by cytokines, may be distinct in disease and provide useful tumour markers. Thus, we have examined the glycosylation of major serum APP in pancreatic cancer (PaC), chronic pancreatitis (CP) and control patients.Experimental design: Using a specific anti-sialyl Lewis X antibody and N-glycan sequencing, we have determined glycosylation changes on alpha-1-acid glycoprotein (AGP), haptoglobin (HPT), fetuin (FET), alpha-1-antitrypsin (AT) and transferrin (TRF).Results: Increased levels of sialyl Lewis X (SLe(x)) were detected on AGP in advanced PaC and CP and on HPT, FET, AT and TRF in CP. An increase in N-glycan branching was detected on AGP and HPT in the advanced stage of PaC and CP and on FET and TRF in the CP. A core fucosylated structure was increased on AGP and HPT only in the advanced PaC patients.Conclusions and clinical relevance: Changes in APP SLe(x) and branching are probably associated with an inflammatory response because they were detected in both advanced PaC and CP patients and these conditions give rise to inflammation. On the contrary, the increase in APP core fucosylation could be cancer associated and the presence of this glycoform may give an advantage to the tumour.