Surface expression of CD63 antigen (AD1 antigen) in P815 mastocytoma cells by transfected IgE receptors
Surface expression of CD63 antigen (AD1 antigen) in P815 mastocytoma cells by transfected IgE receptors
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DOI：
10.1006/bbrc.1996.0304
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发表时间：
1996-02-27
影响因子：
3.1
通讯作者：
Nakanishi, M
中科院分区：
文献类型：
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作者：
Furuno, T;Teshima, R;Nakanishi, M
用共聚焦荧光显微镜观察了抗原刺激后大鼠嗜碱性白血病细胞（RBL-2 H3）表面CD 63抗原的表达。CD 63抗原的表面表达反映了RBL-2 H3细胞的脱颗粒。然后，我们在P815肥大细胞瘤细胞中用转染的IgE受体进行了同样的实验。在具有正常IgE受体的P815细胞中观察到表达，但在具有IgE受体的P815变体细胞中未观察到表达，所述IgE受体缺失β或γ亚基的C-末端胞质结构域。除莠霉素A预处理可阻断IgE受体正常的P815细胞表达。结果表明，β和γ亚基的C-末端胞质结构域的酪氨酸磷酸化是脱粒所必需的。(C)出版社：Academic Press，Inc.
The surface expression of CD63 antigen in rat basuphilic leukemia cells (RBL-2H3) was observed after antigen stimulation by confocal fluorescence microscopy. The surface expression of CD63 antigen reflected the degranulation in RBL-2H3 cells. Then, we did the same experiments in P815 mastocytoma cells with transfected IgE receptors. The expression was observed in P815 cells with normal IgE receptors, but not in P815 variant cells with IgE receptors which were missing a C-terminal cytoplasmic domain of beta or gamma subunit. In addition, the expression in P815 cells with normal IgE receptors was mostly blocked by the pretreatment of herbimycin A. The results suggested that tyrosine phosphorylation of the C-terminal cytoplasmic domains of beta and gamma subunits was essential for degranulation. (C) 1996 Academic Press, Inc.