Photophysiological and photosynthetic complex changes during iron starvation in Synechocystis sp. PCC 6803 and Synechococcus elongatus PCC 7942.
Photophysiological and photosynthetic complex changes during iron starvation in Synechocystis sp. PCC 6803 and Synechococcus elongatus PCC 7942.
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DOI：
10.1371/journal.pone.0059861
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Cockshutt AM
中科院分区：
文献类型：
--
作者：
Fraser JM;Tulk SE;Jeans JA;Campbell DA;Bibby TS;Cockshutt AM
铁是许多参与光合作用的蛋白质复合物的重要组成部分，但由于铁的氧化形式不溶于水，因此环境铁的可用性通常很低。为了适应低环境铁水平，蓝藻经历了许多变化，以平衡其铁预算和减轻铁耗竭的生理影响。我们研究了铁剥夺120小时过程中的关键蛋白质丰度和生理参数的变化，在模型蓝藻聚球藻PCC 7942和集胞藻PCC 6803。铁胁迫诱导蛋白（IsiA）积累到高水平的48小时内的铁剥夺的发病，达到的摩尔比为1042 IsiA：光系统I在聚球藻PCC 7942和1012 IsiA：光系统I在集胞藻PCC 6803。同时，富含铁的复合物细胞色素b6f和光系统I的丰度下降，导致光系统I：光系统II的比例下降。叶绿素荧光分析表明，每个光系统II在聚球藻的电子传递下降，但不是在集胞藻铁耗尽后。我们发现没有证据表明积累的IsiA有助于光系统II复合物的光捕获。
Iron is an essential component in many protein complexes involved in photosynthesis, but environmental iron availability is often low as oxidized forms of iron are insoluble in water. To adjust to low environmental iron levels, cyanobacteria undergo numerous changes to balance their iron budget and mitigate the physiological effects of iron depletion. We investigated changes in key protein abundances and photophysiological parameters in the model cyanobacteria Synechococcus PCC 7942 and Synechocystis PCC 6803 over a 120 hour time course of iron deprivation. The iron stress induced protein (IsiA) accumulated to high levels within 48 h of the onset of iron deprivation, reaching a molar ratio of ∼42 IsiA : Photosystem I in Synechococcus PCC 7942 and ∼12 IsiA : Photosystem I in Synechocystis PCC 6803. Concomitantly the iron-rich complexes Cytochrome b6f and Photosystem I declined in abundance, leading to a decrease in the Photosystem I : Photosystem II ratio. Chlorophyll fluorescence analyses showed a drop in electron transport per Photosystem II in Synechococcus, but not in Synechocystis after iron depletion. We found no evidence that the accumulated IsiA contributes to light capture by Photosystem II complexes.
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影响因子：
7.4
作者：
Joshua, S;Bailey, S;Mullineaux, CW
通讯作者：
Mullineaux, CW
影响因子：
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