Protein partners of KCTD proteins provide insights about their functional roles in cell differentiation and vertebrate development
Protein partners of KCTD proteins provide insights about their functional roles in cell differentiation and vertebrate development
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DOI：
10.1002/bies.201300002
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发表时间：
2013-07-01
期刊：
影响因子：
4
通讯作者：
Baranova, Ancha
中科院分区：
文献类型：
--
作者：
Skoblov, Mikhail;Marakhonov, Andrey;Baranova, Ancha
KCTD家族包括含有具有不同生物学效应的蛋白质的四聚化（T1）结构域。我们鉴定了KCTD家族的一个新成员，BTBD 10。蛋白质-蛋白质相互作用（PPIs）的全面分析使我们能够提出一些关于单个KCTD蛋白的生物学功能的可验证的假设。特别地，我们预测KCTD 20参与AKT-mTOR-p70 S6 k信号级联，KCTD 5以NEK 6和ch-TOG依赖性方式在胞质分裂中起作用，KCTD 10调节RhoA/RhoB通路。发育调节因子KCTD 15抑制AP-2 α，并通过抑制早期脂肪形成促进能量稳态。TNFAIP 1样KCTD蛋白可能通过PCNA泛素化参与复制后DNA修复。KCTD 12可能通过干扰GABAb信号通路抑制胃肠道细胞增殖。KCTD 9作为唯一具有DNA样五肽重复结构域的真核蛋白值得实验关注。不应低估人工管理PPI和分析现有高通量数据的价值。
The KCTD family includes tetramerization (T1) domain containing proteins with diverse biological effects. We identified a novel member of the KCTD family, BTBD10. A comprehensive analysis of protein-protein interactions (PPIs) allowed us to put forth a number of testable hypotheses concerning the biological functions for individual KCTD proteins. In particular, we predict that KCTD20 participates in the AKT-mTOR-p70 S6k signaling cascade, KCTD5 plays a role in cytokinesis in a NEK6 and ch-TOG-dependent manner, KCTD10 regulates the RhoA/RhoB pathway. Developmental regulator KCTD15 represses AP-2 alpha and contributes to energy homeostasis by suppressing early adipogenesis. TNFAIP1-like KCTD proteins may participate in post-replication DNA repair through PCNA ubiquitination. KCTD12 may suppress the proliferation of gastrointestinal cells through interference with GABAb signaling. KCTD9 deserves experimental attention as the only eukaryotic protein with a DNA-like pentapeptide repeat domain. The value of manual curation of PPIs and analysis of existing high-throughput data should not be underestimated.