Cellulose type chiral stationary phase based on reduced graphene oxide@silica gel for the enantiomer separation of chiral compounds
Cellulose type chiral stationary phase based on reduced graphene oxide@silica gel for the enantiomer separation of chiral compounds
复制标题
基于还原氧化石墨烯@硅胶的纤维素型手性固定相用于手性化合物的对映体分离
DOI：
10.1002/chir.22976
复制
发表时间：
2018-08-01
期刊：
影响因子：
2
通讯作者：
Ma, Yulong
中科院分区：
文献类型：
--
作者：
Li, Yuanyuan;Li, Qiang;Ma, Yulong
将氧化石墨烯（GO）通过酰胺键共价连接到硅胶（SiO2）微球表面，得到氧化石墨烯@硅胶（GO@SiO2）微球。然后，将GO@SiO2用肼还原成还原氧化石墨烯@硅胶（rGO@SiO2），并将纤维素衍生物物理包覆在rGO@SiO2表面，制备了高效液相色谱手性固定相（CSP）。在最佳实验条件下，8种苯富集的对映体得到完全分离，反式氧化二苯乙烯的分离度达到4.83。与未键合rGO的空白柱相比，新型CSP的分离性能更好，这是由于rGO的存在与分析物产生了特殊的保留相互作用，如π-π堆积、疏水效应、π-π电子-给体-受体相互作用以及氢键作用。因此，所得CSP对苯富集的对映体表现出特殊的选择性，提高了分离的选择性和效率，rGO与纤维素衍生物在对映体分离中发挥了协同效应。
The graphene oxide (GO) was covalently coupled to the surfaces of silica gel (SiO2) microspheres by amide bond to get the graphene oxide@silica gel (GO@SiO2). Then, the GO@SiO2 was reduced with hydrazine to the reduced graphene oxide@silica gel (rGO@SiO2), and the cellulose derivatives were physically coated on the surfaces of rGO@SiO2 to prepare a chiral stationary phase (CSP) for high performance liquid chromatography. Under the optimum experimental conditions, eight benzene-enriched enantiomers were separated completely, and the resolution of trans-stilbene oxide perfectly reached 4.83. Compared with the blank column of non-bonded rGO, the separation performance is better on the new CSP, which is due to the existence of rGO to produce special retention interaction with analytes, such as pi-pi stacking, hydrophobic effect, pi-pi electron-donor-acceptor interaction, and hydrogen bonding. Therefore, the obtained CSP shows special selectivity for benzene-enriched enantiomers, improves separation selectivity and efficiency, and rGO plays a synergistic effect with cellulose derivatives on enantioseparation.