Membrane potentials, oxidative stress and the dispersal response of bacterial biofilms to 405 nm light
Membrane potentials, oxidative stress and the dispersal response of bacterial biofilms to 405 nm light
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DOI：
10.1088/1478-3975/ab759a
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发表时间：
2020-05-01
期刊：
影响因子：
2
通讯作者：
Waigh, T. A.
中科院分区：
文献类型：
--
作者：
Blee, J. A.;Roberts, I. S.;Waigh, T. A.
大多数慢性感染是由生物膜引起的，生物膜具有比浮游植物更高的抗生素耐药性。紫蓝405 nm光是最近出现的一种新型杀菌剂，但对其抗生物被膜效果的研究有限。我们发现，铜绿假单胞菌和枯草芽孢杆菌生物膜对405 nm光的响应都表现出细胞分散和膜电位超极化。对405 nm光的响应取决于生物膜的生长阶段。活性氧清除剂的使用减少了膜的超极化和生物膜的分散，以响应405 nm的光。这是第一次将膜电位超极化与细菌的光氧化应激和生物膜的分散联系起来。这些结果为膜电位在细菌应激反应中的作用提供了新的见解，并可用于开发基于405 nm光的生物膜处理。
The majority of chronic infections are caused by biofilms, which have higher levels of antibiotic resistance than planktonic growth. Violet-blue 405 nm light has recently emerged as a novel bactericide, but limited studies have been conducted on its effectiveness against biofilms. We found that in response to 405 nm light both Pseudomonas aeruginosa and Bacillus subtilis biofilms exhibited cell dispersal and membrane potential hyperpolarisations. The response to 405 nm light depended on the stage of biofilm growth. The use of reactive oxygen species scavengers reduced membrane hyperpolarisation and biofilm dispersal in response to 405 nm light. This is the first time that membrane potential hyperpolarisations have been linked with photooxidative stress in bacteria and with biofilm dispersal. These results provide a new insight into the role of membrane potentials in the bacterial stress response and could be used in the development of 405 nm light based biofilm treatments.