Enhanced multi-spectral imaging of live breast cancer cells using immunotargeted gold nanoshells and two-photon excitation microscopy
Enhanced multi-spectral imaging of live breast cancer cells using immunotargeted gold nanoshells and two-photon excitation microscopy
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DOI：
10.1088/0957-4484/19/31/315102
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发表时间：
2008-08-06
期刊：
影响因子：
3.5
通讯作者：
Drezek, Rebekah
中科院分区：
文献类型：
--
作者：
Bickford, Lissett;Sun, Jiantang;Drezek, Rebekah
我们展示了使用免疫靶向的金纳米壳作为体外双光子显微镜造影剂的能力。在780 nm的近红外激发下，首次验证了不同尺寸的金纳米壳的双光子发光特性。然后探索了双光子显微镜作为一种工具对标记了抗HER2结合纳米壳的HER2过表达的乳腺癌细胞进行成像的有效性，并将成像结果与正常乳腺细胞进行了比较。在451-644 nm的发射波长范围内，同时检测了五种不同的成像通道。我们的结果表明，在近红外激发下，免疫靶向纳米壳标记的SK-BR-3癌细胞在590-644 nm的发射波长处出现了较好的对比度。在相同的条件下，标记的正常乳腺细胞的发光以及未标记的癌症和正常细胞的自发荧光仍然是不可察觉的。
We demonstrate the capability of using immunotargeted gold nanoshells as contrast agents for in vitro two-photon microscopy. The two-photon luminescence properties of different-sized gold nanoshells are first validated using near-infrared excitation at 780 nm. The utility of two-photon microscopy as a tool for imaging live HER2-overexpressing breast cancer cells labeled with anti-HER2-conjugated nanoshells is then explored and imaging results are compared to normal breast cells. Five different imaging channels are simultaneously examined within the emission wavelength range of 451-644 nm. Our results indicate that under near-infrared excitation, superior contrast of SK-BR-3 cancer cells labeled with immunotargeted nanoshells occurs at an emission wavelength ranging from 590 to 644 nm. Luminescence from labeled normal breast cells and autofluorescence from unlabeled cancer and normal cells remain imperceptible under the same conditions.