Toxoplasma gondii infection in Amami spiny rat on Amami-Oshima Island, Japan
Toxoplasma gondii infection in Amami spiny rat on Amami-Oshima Island, Japan
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DOI：
10.1016/j.ijppaw.2019.06.001
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发表时间：
2019-08-01
影响因子：
1.8
通讯作者：
Ike, Kazunori
中科院分区：
文献类型：
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作者：
Tokiwa, Toshihiro;Yoshimura, Hisashi;Ike, Kazunori
奄美棘鼠（Tokudaia osimensis）是日本鹿儿岛奄美大岛森林中特有的濒危啮齿类动物。2018年7月，在奄美大岛中南部海岸的Yuwandake山步道上发现了一只死亡的成年雄性奄美棘鼠。组织学观察显示肝、肺和心脏中有原生动物感染。针对B1、SAG3、GRA6和ROP 18基因的巢式或半巢式PCR成功地检测了福尔马林固定和石蜡包埋标本中的弓形虫基因组DNA。SAG3、GRA6和ROP 18基因的序列分析表明，在研究样本中检测到的菌株与T.刚地。这是首例确诊的T.刚地感染的一个奄美多刺大鼠。
The Amami spiny rat (Tokudaia osimensis) is an endangered rodent species that is endemic to the forests of Amami-Oshima Island, Kagoshima, Japan. In July 2018, a deceased adult male Amami spiny rat was found on the Yuwandake Mountain Trail on the south-central coast of Amami-Oshima Island. Histopathological observations revealed protozoan infections in the liver, lungs, and heart. Nested or semi-nested PCRs targeting the B1, SAG3, GRA6, and ROP18 genes successfully detected the genomic DNA of Toxoplasma gondii in the formalin fixed and paraffin-embedded specimen. Sequence analyses of the SAG3, GRA6, and ROP18 genes suggested that the strain detected in the study specimen was related to the type II strain of T. gondii. This is the first confirmed case of T. gondii infection in an Amami spiny rat.