Quantification of Free 2-Furfurylthiol in Coffee Brew Using a Prefabricated Coffee Model
Quantification of Free 2-Furfurylthiol in Coffee Brew Using a Prefabricated Coffee Model
复制标题
使用预制咖啡模型定量咖啡冲泡中的游离 2-糠基硫醇
作者：
Zhenchun Sun;K. Hayat;Jingyang Yu;E. Karangwa;E. Duhoranimana;Xiaoming Zhang;S. Xia
建立了一种可靠、灵敏的测定咖啡中游离2-糠硫醇（2-FFT）的方法。采用半胱氨酸添加与真空蒸馏相结合的方法，制备了具有痕量2-FFT和与咖啡冲泡物相似的理化性质的咖啡模型，用于标准曲线的构建。在模型制备过程中，结合形式的2-FFT被释放为游离2-FFT，并通过真空蒸馏从咖啡冲泡物中除去。对方法的灵敏度、精密度、准确度和选择性进行了评价。定量限（LOQ）为3.0 μg/L。变异系数（CV）为7.1%。在22.6、135.8和181 μg/L的加标浓度下，平均回收率为86.8-106.2%。对罗布斯塔和阿拉比卡烘焙咖啡豆制备的咖啡冲泡物进行了分析，结果表明罗布斯塔咖啡豆制备的咖啡冲泡物的游离2-FFT含量（20.94 μg/L）分别高于云南阿拉比卡咖啡豆（11.34 μg/L）和哥伦比亚阿拉比卡咖啡豆（15.33 μg/L）。该方法可用于咖啡中游离2-FFT含量的测定和基于游离2-FFT含量的感官分析。
A reliable and sensitive method was developed to quantify the free 2-furfurylthiol (2-FFT) in coffee brew. Using cysteine addition combined with vacuum distillation, a coffee model with trace 2-FFT and similar physicochemical properties as coffee brew was prepared for standard curve construction. During the model preparation, 2-FFT in bound form was released to free 2-FFT and removed from coffee brew through vacuum distillation. The method sensitivity, precision, accuracy and selectivity were evaluated. The limit of quantification (LOQ) was 3.0 μg/L. The coefficient of variation (CV) was 7.1%. The average recovery rates were 86.8–106.2% at spiked concentrations of 22.6, 135.8 and 181 μg/L. Coffee brews prepared using Robusta and Arabica roasted coffee beans were analyzed, and results showed that coffee brew from Robusta coffee beans had a greater amount of free 2-FFT (20.94 μg/L) than Arabica coffee beans from Yunnan (11.34 μg/L) and Columbia (15.33 μg/L), respectively. This method would be beneficial to investigate the free amount of 2-FFT in coffee brew and the sensorial assay based on free 2-FFT concentration.