Evaluating impacts of Ralstonia eutrophaKT-1 injected into a trichloroethylene contaminated site on indigenous bacterial populations in groundwater
Evaluating impacts of Ralstonia eutrophaKT-1 injected into a trichloroethylene contaminated site on indigenous bacterial populations in groundwater
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评估注入三氯乙烯污染场地的富养罗尔斯通氏菌 KT-1 对地下水中本地细菌种群的影响
DOI：
10.11532/proes1992.39.333
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发表时间：
2002
影响因子：
3.5
通讯作者：
T. Iizumi
中科院分区：
文献类型：
--
作者：
Kanji Nakamura;H. Ishida;T. Iizumi
摘要：真养罗尔斯通氏菌KT-1是一株能在苯酚和甲苯上生长的细菌。经苯酚或甲苯诱导后，KT-1菌株也能降解三氯乙烯。将约2 kg（干重）甲苯诱导的菌株KT-1注入TCE污染场地的地下水中，以降解TCE。注入过程完成后，地下水被抽了51天。采用最大概然数（MPN）法对该时期地下水中苯酚降解菌数量进行了监测。通过16 S rDNA分子生物学方法分析了KT-1对地下水细菌种群的影响。16 S rDNA克隆分析表明，实验中获得的克隆中有68%属于变形菌门α或β。末端限制性片段长度多态性（T-RFLP）分析的PCR扩增的16 S rDNA的结果表明，土著细菌亚硝化单胞菌和固氮菌群从地下水中消失后，注射菌株KT-1。然而，在抽水18天后，它们再次被观察到为优势种。采用多维标度（MDS）对T-RFLP数据进行统计分析，以评价细菌种群的变化。结果表明，KT-1菌株干扰后的土著细菌种群在51天内恢复良好。
ABSTRACT, Ralstonia eutropha KT-1 is a bacterium that can grow on phenol or toluene. Strain KT-1 is also able to degrade trichloroethylene (TCE) after the induction with phenol or toluene. Approximately 2 kg (dry weight) of strain KT-1 induced by toluene was injected into groundwater at a TCE-contaminated site to degrade TCE. After the injection process was completed, the groundwater was pumped up for 51 days. Number of phenol-degrading bacteria in the groundwater was monitored by most probable number (MPN) method during that period. Changes of bacterial populations in the groundwater were also analyzed by molecular methods based on 16S rDNA to evaluate impacts of strain KT-1 injected on indigenous bacterial population. Analysis of 16S rDNA clone showed that 68% of clones obtained during the experiment belonged to Proteobacteria Alpha or Beta. Terminal restriction fragment length polymorphisms (T-RFLP) analysis of PCR-amplified 16S rDNAs indicated that indigenous bacteria phylogenetically close to Nitrosomonas and Azoarcus group disappeared from the groundwater after the injection of strain KT-1. However, they were observed again as dominants after 18 days of pumping up. A statistical analysis, multi-dimensional scaling (MDS), was applied to the data of T-RFLP in order to evaluate the changes of bacterial populations. The results showed that indigenous bacterial populations perturbed by strain KT-1 were well restored in 51 days.