Quantitative assessment of rat bone regeneration using complex master-slave optical coherence tomography
Quantitative assessment of rat bone regeneration using complex master-slave optical coherence tomography
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DOI：
10.21037/qims.2019.05.03
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发表时间：
2019-05-01
影响因子：
2.8
通讯作者：
Podoleanu, Adrian Gh
中科院分区：
文献类型：
--
作者：
Luca, Ruxandra Elena;Todea, Carmen Darinca;Podoleanu, Adrian Gh
背景：口腔植入学中对硬组织和软组织的需求确定了使用用作患者天然骨骼的取代基的不同替代材料来增加骨骼体积及其质量的方法。 In addition, laser radiation can be used to accelerate the repair of fractures and to produce an increased volume of formed callus, as well as an increased bone mineral density.Methods: The aim of this work is to evaluate the capability of an in-house developed multimodal complex master slave (CMS) enhanced swept source (SS) optical coherence tomography (OCT) imaging instrument to analyze the increase in the quantity and the improvement of the quality of使用低水平激光疗法（LLLT）的新形成骨。骨形成在活大鼠头骨的颅骨部分中的5 mM圆柱形缺陷中进行定量评估。样品分为三个研究组：A，一个阴性对照组，为此研究了缺陷的自然愈合过程； B，一个阳性对照组，用于牛移植物用于刺激骨骼形成，C（研究组）在整个愈合期间都添加了牛移植物，其中牛移植物被添加到所产生的缺陷和LLLT中。在14、21和30天后处死动物，并使用多模式CMS/SS-OCT仪器对样品进行成像：该方法允许通过两个垂直的横截面和九个在可调的深度以可调的深度拍摄到样品的垂直横截面，同时监测骨组织。带有轴向分辨率的全局图像允许在组织上感兴趣的区域定位系统的视野。使用天然骨骼，人造骨移植和新形成的骨骼之间的亮度差异来完成对骨形成过程的定量评估。结论：C组的新成型骨骼的新成型骨比组更高，从这一观点中，这是在这一观点中比这两次的第三季，因此，这是在这两个周期中，因此，这是在两天的时间上分析的，这是最重要的，这是在21天的时间上，大约是在这一数量的情况下，这是最重要的差异。间隔分析。 30天后，随着它们开始填补可用空隙，骨骼的体积往往会靠近。该研究表明，OCT可以定量评估LLLT对骨再生的积极影响。
Background: The need for hard and soft tissues in oral implantology determined the development of methods and techniques to increase bone volume and their quality with different alternative materials used as substituents of patient's natural bone. In addition, laser radiation can be used to accelerate the repair of fractures and to produce an increased volume of formed callus, as well as an increased bone mineral density.Methods: The aim of this work is to evaluate the capability of an in-house developed multimodal complex master slave (CMS) enhanced swept source (SS) optical coherence tomography (OCT) imaging instrument to analyze the increase in the quantity and the improvement of the quality of newly-formed bone using low level laser therapy (LLLT). Bone formation is quantitatively assessed in 5 mm cylindrical defects made in the calvaria part of the skull of living rats. Samples are divided in three study groups: A, a negative control group, for which the natural healing process of the defect is investigated; B, a positive control group, for which bovine graft is used to stimulate bone formation, and C, a study group, in which bovine graft is added to the created defects and LLLT is applied throughout the entire healing period. The animals are sacrificed after 14, 21, and 30 days, and the samples are imaged using the multimodal CMS/SS-OCT instrument.Results: The method allows for the simultaneous monitoring of the bone tissue via two perpendicular cross-sections and nine en-face images taken at adjustable depths into the sample. A global image with course axial resolution allows for the positioning of the field-of-view of the system on the area of interest on the tissue. The quantitative assessment of the process of bone formation is completed using the differences in brightness between the native bone, the artificial bone graft, and the newly-formed bone.Conclusions: Group C is demonstrated to have a higher volume of newly-formed bone than Group B, which is better from this point of view than Group A. By analyzing the evolution of this volume of new bone in time, the most significant difference was after 21 days, therefore approximately after two thirds of the total time interval analyzed. After 30 days, the volumes of bone tend to move closer, as they begin to fill the available gap. The study demonstrates that OCT can assess quantitatively the positive impact of LLLT on bone regeneration.