Detecting Bacillus cereus spores on a mail sorting system using Raman spectroscopy
Detecting Bacillus cereus spores on a mail sorting system using Raman spectroscopy
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DOI：
10.1002/jrs.1111
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发表时间：
2004-01-01
影响因子：
2.5
通讯作者：
Fenerty, G
中科院分区：
文献类型：
--
作者：
Farquharson, S;Grigely, L;Fenerty, G
研究了拉曼光谱法检测通过邮件分拣系统的炭疽孢子的能力。将泵连接到商业分选机上的现有真空歧管，并将设计用于捕获0.5-3 μ m颗粒的过滤器在线放置。将含有0.23g蜡状芽孢杆菌孢子（炭疽芽孢杆菌替代物）的标准商业信件放置在20封信件的堆叠中并通过系统。过滤器的拉曼光谱通过与孢子核相关的主导的吡啶二甲酸钙拉曼光谱带将捕获的材料肯定地鉴定为细菌孢子。使用400 mW的785 nm激光激发1 s采集的检测限估计为4.5 mg。讨论了基于拉曼光谱的系统检测和防止含有克级炭疽孢子的字母的分布的能力。版权所有（C）2004约翰威利父子有限公司。
The ability of Raman spectroscopy to detect anthrax-causing spores as they pass through a mail sorting system was investigated. A pump was connected to an existing vacuum manifold on a commercial sorter, and a filter designed to capture 0.5-3 mum particles was placed in-line. A standard business letter containing 0.23 g of Bacillus cereus spores, a Bacillus anthracis surrogate, was placed in a stack of 20 letters and passed through the system. Raman spectra of the filter positively identified the captured material as bacterial spores by the dominant calcium dipicolinate Raman spectral bands associated with the spore core. A limit of detection, using 400 mW of 785 nm laser excitation for a 1-s acquisition, is estimated at 4.5 mg. The ability of a Raman spectroscopy based system to detect and prevent the distribution of a letter containing gram levels of anthrax spores is discussed. Copyright (C) 2004 John Wiley Sons, Ltd.