Molecular cloning and mRNA tissue expression of thyroid hormone receptors in yellow catfish Pelteobagrus fulvidraco and Javelin goby Synechogobius hasta.
Molecular cloning and mRNA tissue expression of thyroid hormone receptors in yellow catfish Pelteobagrus fulvidraco and Javelin goby Synechogobius hasta.
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DOI：
10.1016/j.gene.2013.12.035
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发表时间：
2014-02
期刊：
影响因子：
3.5
通讯作者：
Qi-Liang Chen;Zhi Luo;Xiao-Ying Tan;Ya-Xiong Pan;Jia-Lang Zheng;M. Zou
中科院分区：
文献类型：
--
作者：
Qi-Liang Chen;Zhi Luo;Xiao-Ying Tan;Ya-Xiong Pan;Jia-Lang Zheng;M. Zou
甲状腺激素在包括鱼类在内的脊椎动物的许多生理功能中起着关键作用。它们的作用是由甲状腺激素受体（TRs）介导的，TRs是核激素受体超家族的成员。本研究克隆了黄颡鱼和标枪虾虎鱼TR的全长cDNA序列，并测定了它们的mRNA组织表达谱。InP。结果表明，TRα和TRβ的cDNA全长分别为1789和1848 bp，分别编码401和378个氨基酸残基。此外，还发现了一种TRβ剪接变体（命名为P. fulvidraco-TRβv），含有60 bp的插入片段。在Hasta中，编码TRαA、TRαB和TRβ的cDNA分别为1827、2295和2258 bp，分别编码401、409和393个氨基酸残基的多肽。系统发育分析表明，TRα和TRβ cDNA与其他脊椎动物的对应序列聚为两个独立的簇，并且两个TRα序列分别聚为两个簇，这表明这两个TRα可能来自于在硬骨鱼特异性基因组复制事件中产生的旁系同源基因。在取样的各种组织中检测到所有TR mRNA。结果表明，在同一时间点，P.黄颡鱼脑的含量最高，其次是肝，心、肠、肌肉、鳃和脾的含量最低。而在短刺梭子蟹中，TRαA、TRαB和TRβ在脑中的表达量最高，在肌肉中的表达量最低。目的：克隆和鉴定黑胸草TR基因，并对其mRNA组织表达进行分析。fulvidracoandS. hasta为了解它们在这两种鱼类中的生物作用迈出了第一步。
Thyroid hormones (THs) play a pivotal role in many physiological functions in vertebrates, including fish. Their effects are mediated by thyroid hormone receptors (TRs), which are members of the nuclear hormone receptor superfamily. In this study, full-length cDNA sequences of TRs from yellow catfishPelteobagrusfulvidracoand Javelin gobySynechogobius hastawere cloned and their mRNA tissue expression profiles were determined. InP. fulvidraco, the validated cDNAs encoding for TRα and TRβ were 1789 and 1848 bp in length, encoding peptides of 401 and 378 amino acid residues, respectively. In addition, a TRβ spliced variant (namedP. fulvidraco-TRβv), containing a 60-bp insertion, was detected. InS. hasta, cDNAs encoding for TRαA, TRαB and TRβ were 1827, 2295 and 2258 bp in length, encoding peptides of 401, 409 and 393 amino acid residues, respectively. The phylogenetic analysis revealed that TRα and TRβ cDNAs grouped into two separate clusters with other vertebrate counterparts and two TRα sequences grouped separately, suggesting that the two TRαs derived from paralogous genes that might arise during a teleost-specific genome duplication event. All TR mRNAs were detected in various tissues sampled. The mRNA levels of both TRα and TRβ fromP. fulvidracowere the highest in brain, followed by liver, and lowest in heart, intestine, muscle, gill and spleen. However, inS. hasta, TRαA, TRαB and TRβ showed the highest mRNA levels in brain and lowest in muscle. Identification and mRNA tissue expression of TR genes fromP. fulvidracoandS. hastaprovide an initial step towards understanding their biological roles in the two fish species.