Determination of trans-resveratrol in plasma by HPLC
Determination of trans-resveratrol in plasma by HPLC
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DOI：
10.1021/ac9808831
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发表时间：
1999-02-01
影响因子：
7.4
通讯作者：
Planas, JM
中科院分区：
文献类型：
--
作者：
Juan, ME;Lamuela-Raventós, RM;Planas, JM
反式-白藜芦醇(反式-3，5，4‘-三羟基二苯乙烯)是一种存在于葡萄、葡萄酒和花生中的酚类化合物，据报道，它对健康有好处，包括抗癌和预防心血管疾病。尽管它很重要，但人们对其在人类和动物中的生物利用度知之甚少，这一评估的基本步骤包括测量血液中的这种二苯乙烯类化合物。在本工作中，建立了一种简单快速的二极管阵列紫外检测的高效液相色谱方法。血浆中的白藜芦醇用CLS小柱固相萃取纯化。样品用水和甲醇-水(25：75v/v)洗脱，反式白藜芦醇用甲醇洗脱。收集的馏分在氮气中蒸发，并用高效液相色谱法进行分析。方法线性相关良好，检出限为20微克/L，精密度好，变异系数为2.85%，可用于大鼠血浆中反式白藜芦醇的检测。通过这种程序，可以很好地分离反式白藜芦醇，从而可以快速分析样品，以进行吸收、分布和代谢研究。
trans-Resveratrol (trans-3,5,4'-trihydroxystilbene), a phenolic compound present in grapes, wines, and peanuts, has been reported to have health benefits including anticarcinogenic effects and protection against cardiovascular diseases. Despite its importance, little is known about its bioavailability in both humans and animals, A fundamental step for this evaluation consisted in measuring-this stilbene in blood, In the present work, a simple and rapid HPLC method with diode array-UV detection has been developed. Resveratrol contained in plasma was purified by solid-phase extraction using a Cls cartridge. The sample was rinsed with water and methanol-water (25:75 v/v), and trans-resveratrol was finally eluted with methanol. The collected fraction was evaporated under nitrogen and analyzed by HPLC. The method was validated by obtaining a linear correlation, a detection limit of 20 mu g/L, and a good precision with a coefficient of variation of 2.85%, trans-Resveratrol administered orally to rats was detected in plasma. With this procedure, excellent separation of trans-resveratrol is achieved, thus allowing a rapid analysis of the sample for absorption, distribution, and metabolism studies.