The low affinity binding site for the cocaine analog, WIN 35,428 is an artifact of freezing caudate tissue.
The low affinity binding site for the cocaine analog, WIN 35,428 is an artifact of freezing caudate tissue.
复制标题
可卡因类似物 WIN 35,428 的低亲和力结合位点是冷冻尾状核组织的产物。
DOI：
10.1016/0024-3205(92)90329-n
复制
发表时间：
1992
期刊：
影响因子：
6.1
通讯作者：
Aloyo,VJ
中科院分区：
文献类型：
--
作者：
Kirifides,AL;Harvey,JA;Aloyo,VJ
研究了可卡因类似物[3H] win35,428在大鼠和家兔尾状核中的结合。在新鲜组织制备的膜中，[3WIN 35,428结合的特点是具有一个高亲和力位点，家兔和大鼠的Kdof分别为2.5 nM和5.3 nM。相比之下，[3H] win35,428与冷冻组织（保存在- 70°C）制备的膜结合显示出两个结合位点，一个高亲和力位点与新鲜组织膜相似，另一个低亲和力位点的Kdof为家兔39 nM，大鼠35 nM。低亲和力的win35,428结合位点，对大鼠65 nM。亲和的win35,428结合位点仅在来自冷冻组织的膜中观察到，这表明它是在冷冻/解冻过程中产生的产物。
Binding of the cocaine analog [3H] WIN 35, 428 was investigated in rat and rabbit caudate. In membranes prepared from fresh tissue, [3WIN 35, 428 binding was characterized by a single high affinity site with a Kdof 2.5 nM for the rabbit and 5.3 nM for the rat. In contrast, [3H] WIN 35, 428 binding to membranes prepared from frozen tissue (stored at −70° C) revealed two binding sites, a high affinity site similar to the one observed in membranes from fresh tissue and a low affinity site with a Kdof 39 nM for the rabbit and 35 nM for the rat. The low affinity WIN 35, 428 binding site and 65 nM for the rat. The affinity WIN 35, 428 binding site was observed only in membranes derived from frozen tissue, suggesting that it was an artifact produced by the freezing/thawing process.