Phosphorylation of histone H3 by Haspin regulates chromosome alignment and segregation during mitosis in maize
Phosphorylation of histone H3 by Haspin regulates chromosome alignment and segregation during mitosis in maize
复制标题
Haspin 对组蛋白 H3 的磷酸化调节玉米有丝分裂过程中的染色体排列和分离
DOI：
10.1093/jxb/eraa506
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发表时间：
2021-02-24
影响因子：
6.9
通讯作者：
Han, Fangpu
中科院分区：
文献类型：
--
作者：
Liu, Yang;Wang, Chunhui;Han, Fangpu
在人类细胞中，haspin介导的组蛋白H3苏氨酸3 （H3T3）磷酸化促进了染色体乘客复合物的着丝粒定位，从而确保了着丝点-微管的适当附着。Haspin还与PDS5内聚素相关因子B （Pds5B）结合，拮抗Wings apart-like protein homolog (Wapl)-Pds5B相互作用，从而阻止Wapl在有丝分裂过程中释放着丝粒内聚。然而，Haspin在植物染色体分离中的作用尚不清楚。在这里，我们发现在玉米（Zea mays）有丝分裂细胞中，ZmHaspin在中期和后期定位于着丝粒，而在减数分裂期间定位于端粒。这些结果表明，ZmHaspin在有丝分裂和减数分裂过程中起着不同的作用。敲除ZmHaspin导致H3T3磷酸化和组蛋白H3丝氨酸10磷酸化降低，有丝分裂时染色体排列和分离缺陷。这些证据表明，Haspin通过人类H3T3磷酸化机制调节植物的染色体分离。植物Haspin蛋白缺乏结合Pds5B和异染色质蛋白1所需的RTYGA和PxVxL基序，并且在敲除ZmHaspin的植物中未检测到明显的内聚缺陷。综上所述，这些结果强调了Haspin蛋白在植物和动物细胞分裂中的保守但略有不同的作用。
In human cells, Haspin-mediated histone H3 threonine 3 (H3T3) phosphorylation promotes centromeric localization of the chromosomal passenger complex, thereby ensuring proper kinetochore-microtubule attachment. Haspin also binds to PDS5 cohesin-associated factor B (Pds5B), antagonizing the Wings apart-like protein homolog (Wapl)-Pds5B interaction and thus preventing Wapl from releasing centromeric cohesion during mitosis. However, the role of Haspin in plant chromosome segregation is not well understood. Here, we show that in maize (Zea mays) mitotic cells, ZmHaspin localized to the centromere during metaphase and anaphase, whereas it localized to the telomeres during meiosis. These results suggest that ZmHaspin plays different roles during mitosis and meiosis. Knockout of ZmHaspin led to decreased H3T3 phosphorylation and histone H3 serine 10 phosphorylation, and defects in chromosome alignment and segregation in mitosis. These lines of evidence suggest that Haspin regulates chromosome segregation in plants via the mechanism described for humans, namely, H3T3 phosphorylation. Plant Haspin proteins lack the RTYGA and PxVxL motifs needed to bind Pds5B and heterochromatin protein 1, and no obvious cohesion defects were detected in ZmHaspin knockout plants. Taken together, these results highlight the conserved but slightly different roles of Haspin proteins in cell division in plants and in animals.