Variation of rhizosphere bacterial community in watermelon continuous mono-cropping soil by long-term application of a novel bioorganic fertilizer
Variation of rhizosphere bacterial community in watermelon continuous mono-cropping soil by long-term application of a novel bioorganic fertilizer
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长期施用新型生物有机肥对西瓜连作土壤根际细菌群落的变化
DOI：
10.1016/j.micres.2013.10.004
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发表时间：
2014-01-01
影响因子：
6.7
通讯作者：
Shen, Qirong
中科院分区：
文献类型：
--
作者：
Ling, Ning;Deng, Kaiying;Shen, Qirong
开发了一种新型生物有机肥（BIO）的施用方法，以提高其对枯萎病的生物防治效果（Ling et al. 2010）。但长期施用该药剂对西瓜枯萎病的防治效果及微生物区系的变化尚不清楚。为了阐明这一点，进行了为期4年的单作西瓜盆栽试验。结果表明，虽然各处理的发病率均随连作年限的增加而增加，但无论是苗圃还是盆栽，BIO处理的发病率始终保持最低水平。实时荧光定量PCR结果表明，多粘类芽孢杆菌的种群数量随连作年限的增加而减少，但在所有季节中，无论是苗圃还是盆栽，BIO处理的多粘类芽孢杆菌种群数量均高于其他处理。随着连作年限的增加，病原菌FON的丰度增加，但以BIO处理的增加幅度最小。DGGE图谱显示，单作西瓜4年后，细菌多样性有所减弱，但在育苗期和移栽期连续施用BIO后，细菌多样性变化最小。454焦磷酸测序结果表明，两种处理的优势菌门均为厚壁菌门（Firmicutes）、变形菌门（Proteobacteria）和放线菌门（Actinobacteria），连续施用BIO后，芽孢杆菌（Bacillus）、类芽孢杆菌（Paenibacillus）、盐囊菌（Haliangium）、链霉菌（Streptomyces）等有益菌的数量明显增加。这些结果在一定程度上证实了在育苗期和移栽期连续施用BIO可以通过调控根际细菌多样性来有效抑制西瓜枯萎病。这些结果为如何调控土壤微生物群落到一个适宜的水平以保持植物健康进而控制土传病害提供了一定的线索。(C)2013 Elsevier GmbH. All rights reserved.
The application method for a novel bioorganic fertilizer (BIO) was developed to improve its biocontrol efficacy of Fusarium wilt (Ling et al. 2010). However, its efficacy on controlling Fusarium wilt and the variations of microbial community after long-term application for watermelon production had not been elucidated. To clarify, a 4-years pot experiment of mono-cropping watermelon was conducted. The results revealed that though the disease incidences were increased in all treatments with the increase of continuous cropping years, the treatment of BIO application both in nursery and pot soil always maintained the lowest disease incidence. The real-time PCR results showed that the population of Paenibacillus polymyxa was decreased with continuous cropping years, but in all seasons, the treatment with BIO application both in nursery and pot soil had a highest population of P. polymyxa than the other treatments. On the other hand, the abundance of the pathogen FON was increased with the increase of continuous cropping years and the lowest rate of increase was found by BIO application in both nursery and pot soil. DGGE patterns showed that the bacterial diversity was weakened after mono-cropping of watermelon for 4 years, but the consecutive applications of BIO at nursery and transplanting stage resulted in the minimal change of bacterial diversity. More detailed differences on bacterial diversity between control and double application of BIO treatment after 4-years monoculture were analyzed by 454 pyrosequencing, which showed the dominant phyla found in both samples were Firmicutes, Proteobacteria and Actinobacteria, and the consecutive applications of BIO recruited more beneficial bacteria than control, such as Bacillus, Paenibacillus, Haliangium, Streptomyces. Overall, these results, to a certain extent, approved that the consecutive applications of BIO at nursery and transplanting stage could effectively suppress watermelon Fusarium wilt by regulating the rhizosphere bacterial diversity. These results could give some clues that how to regulate the soil microbial community to an appropriate level which can keep the plant healthy and thus control the soil-borne diseases. (C) 2013 Elsevier GmbH. All rights reserved.